| Zang Yinchen,because of its pharmacological activities of constituents amaroswerin,oleanolic acid and mangiferin,such as antibacterial,antitumor,antibiosis activity.We used the whole plants of Zang Yinchen were air-dried.The antitumor activity were studied.?1?The research of the chemical compositions of Zang YinchenWe obtained eighteen compounds from EtOAc and n-BuOH extract:2-Phenyle-thyl-?-D-glucoside?1?,Orcinol-?-D-glucoside?2?,Shamimin?3?,Amaroswerin?4?,Orcinol?5?,Mangiferin?6?,Decussatin?7?,Bellidtfolin?8?,1,3,7,8-tetrahydroxyxan-thone?9?,Desmethylbellidifolin?10?,Protocatechoic acid?11?,1,7-dihydroxy-3,8-di-methoxyxanthone?12?,1,8-dihydroxy-3,5-dimethoxyxanthone?13?,1-hydroxy-3,5-d-imethoxyxanthone?14?,Swertiamarine?15?,Telephioidin?16?,1,3,8-trihydroxy-5-m-ethoxyxanthone?17?,Methylswertianin?18?.Compounds 1-3,5,11 and 16 were isolated from the genus Swertia for the first time.The antioxidant activities showed that 10,13 exhibited strong biological activities against DPPH radical,and compounds 10,12 and 13 demonstrated powerful activities against ABTS radical.?2?The research of the anti-cancer activities of Zang Yinchen in vitroThe MTT results showed that SMF50,70,100 can inhibited MGC-803,SGC-7901,BGC-823 cells production,Flow cytometric showed that SMF keeps the cell cycle in S phase.Further study found that SMF70,100 decreased the mitochondrial membrane potential,increased intracellular ROS and Ca2+concentration.?3?The research of the anti-cancer activities of Zang Yinchen in vivoWe studied the acute toxicity the results showed that the SMF70 belongs to the low-toxic or non-toxic level.Subcutaneous transplantation tumor and B16-F10pulmonary metastasis in vivo,compared with the blank control group.It may be an effective and potential anti-tumor medicine. |
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