The Osteogenetic Activity Of 12b-hydroxy-des-D-garcigerrin A In Vitro

Garcinia xanthochymus is a traditional Dai Herbal Meidicine,which often used to treat annealing heat,swelling and pain,rheumatism,traumatic injury and fracture in clinics.Currently,most of the research about Garcinia xanthochymus focused on extraction and purification of compounds from it,however,the bioactivities of the components are not known.In this study,we investigate the effect of 12b-hydroxy-des-D-garcigerrin A(bHGA),one extract from Garcinia xanthochymus,on osteoblasts cultured in vitro,to identify whether bHGA has osteogenic biological activity,which might provide information on the development of medicine from bHGA.In this study,the effect of bHGA on proliferation,ALP activity,osteoblast mineralization and the expression of osteogenic gene in MC3T3-E1 was investigated.The Alamar Blue assay showed that 16?mol/L of bHGA had cytotoxicity to osteoblasts.Thus,we selected 2 ?mol/L,4 ?mol/L and 8 ?mol/L of bHGA in the following study.Result showed that the ALP activity of osteoblasts was inhibited by bHGA for 8 days-treatment,but was not affected for long-term treatment(16 days-teratment).The Alizerin Red Stain result showed that 4 ?mol/L,8 ?mol/L could obviously promote cell matrix mineralization.Quantitative PCR results showed that the mRNA expression of ALP,Runx2 and Osterix was inhibited,while the expression of Col-1?OCN was obviously promoted in the whole treatment period.The expression of BSP?BMP2 was enhanced after long-term treatment with bHGA.It is indicated that bHGA might promote extracellular matrix maturation and bone matrix mineralization.In conclusion,bHGA could promote bone matrix formation and extrcellular matrix mineralization.