The Influence Of Closed Irrigation Of HACC On Ischemia/Reperfusion Injury Of Graft Flap In Rats

To investigate the effects of quaternary ammonium salt of Chitosan combined with closed irrigation on ischemia-reperfusion injury(Ischemia/Reperfusion,I/R)in rat skin flap.Methods:1.Experimental animal grouping and model preparation:45 adult male SD rats were stochastically assigned to 3 groups:sham-operated group,I/R group and Chitosan group.Before operation,collection of arterial blood for testing PCT and hs-CRP were used as basic values.Right low abdominal island flap which fed by the superficial epigastric artery were created in rats of all three groups.Blocked the arterial blood flow by vascular clamps for 6 hours to establish ischemia-reperfusion model of flaps.2.Experimental method:After operation,flush and dress the wound with 10%saline of sham-operated group and I/R group.Quaternary ammonium salt of Chitosan combined with closed irrigation were used in Chitosan group.Tissue culture was histologically observed at 3,5,7 days after reperfusion.Gather issue to make the SOD,MDA and PCT,hs-CRP testing,grossly observation and count the flap survival rate.3.Testing index:Histopathology observation.Detect the SOD,MDA,PCT,hs-CRP in every group by relevent kits.Calculate the surviving rate of flaps 7 days after.Results:1.Compared with I/R group,less neutrophil infiltration was observed in Chitosan group.2.PCT and hs-CRP:The PCT and hs-CRP level of the I/R group after modeling for 3days,5days were significantly increased There were statistically significant differences(P<0.05);Compared with the sham-operated group,the PCT and hs-CRP level of the Chitosan group after modeling for 3days,5days were significantly increased.There were statistically significant differences(P<0.05);Compared with the I/R group,the PCT and hs-CRP level of the Chitosan group after modeling for 3days,5days were significantly induced.There were statistically significant differences(P<0.05);after modeling for 7 days,the values between all groups were no statistically significant differences(P>0.01).3.SOD and MDA:Compared with the sham-operated group,the SOD level of the I/R group after modeling for 3days,5days,7days were significantly induced.MDA level were significantly increaesd;To Compared with the sham-operated group,the SOD level of the Chitosan group after modeling for 3days,5days,7 days were significantly induced.MDA level were significantly increaesd;Compared with the I/R group,the SOD level of the Chitosan group after modeling for 3days,5days,7days were significantly increased.MDA level were significantly induced.;They all have statistically significant differences(P<0.05).4.The Chitosan group's flaps survival rate was(85.34±10.02)%,which was significantly greater than the I/R group,in which only(41.11±14.17)%(P<0.05).Conclusions:By quaternary ammonium salt of Chitosan combined with closed irrigation can significantly improve flap survival,reduce I/R in transplanted rats' skin flaps.