Expression And Significance Of IL-17 And Bcl-2 On Conjunctiva Endepidermis Cells In Diabetic Mellitus Patients

Objective:Objective to investigate the expression of IL-17 and Bcl-2 in conjunctival epithelial cells of diabetic patients and its significance.Methods:Diabetic patients were randomly selected from January 2016 to October in our hospital(diabetes group,124 cases)and healthy people(normal control group,98 cases),two groups Schirmer test(Schirmer I test),break-up time(Break up time,BUT),corneal fluorescent staining and impression cytology;using immunohistochemical staining the expression of IL-17 and Bcl-2 detection of conjunctival epithelial cells and conjunctival epithelial cells;apoptosis detection by flow cytometry.Results:1.Compared with the normal control group,diabetic group BUT<10s and Schirmer 's<10mm proportion was significantly increased,the difference was statistically significant(P<0.01),corneal fluorescein staining was significantly stronger than the normal control group(P<0.01);2.Compared with the normal control group,diabetic group BUT,Schirmer 's was negatively related to the expression of apoptosis index and conjunctival epithelial cells and IL-17 and Bcl-2(R=-0.570,P<0.01,R=-0.559,P<0.01;R=-0.614,P<0.01,R=-0.651,P<0.01 FL),and the conjunctiva related expression was apoptosis index of skin cells and IL-17 and Bcl-2(R=0.825,P<0.01;R=0.798,P<0.01);3.Compared with the normal control group,the apoptosis index of conjunctival epithelial cells in diabetic group was positively correlated with the expression of IL-17 and Bcl-2(R=0.445,P<0.01,R=0.548,P<0.01);4.Compared with the normal control group,the staining degree of IL-17 and Bcl-2 in conjunctival epithelial cells of diabetic group was significantly higher than that in normal control group(P<0.01;P<0.01);5.Compared with the normal control group,the apoptosis index of conjunctival epithelial cells in the diabetic group was significantly higher than that in the control group,the difference was statistically significant(P<0.01).Conclusion:1.The expression of IL-17 and Bcl-2 in conjunctival epithelial cells of diabetic patients is obviously increased,which can be used as one of the clinical indicators to judge ocular surface and tear film status.2.The up regulation of IL-17 and Bcl-2 expression is closely related to the onset of Xerophthalmia in diabetes mellitus.