Functions Of MiRNAs In Tumor:miR-129 Influences GCTB By Targeting RANK And MiR-215 Retrains Lung Cancer By Targeting Ral

microRNAs(miRNAs)are small(19-24 nucleotides)non-coding RNA molecules that were first discovered at nematos in 1993.miRNAs act as endogenous suppressors of gene expression by binding to 3'-UTR of the target gene's mRNAs.A large number of mi RNAs including miR-31,miR-86,miR-183 and miR-221/222 were reported playing an important role in many biological process of tumors,such as poliferation,differentiation,apoptosis and invasion.More and more researchs are carried on the connection between miRNAs and tumor.In this study,wo focused on giant cell tumor of bone(GCTB)and lung cancer,We studied how miR-129 influences it's target RANK and how miR-125 influences the target Ral to inhibit the tumor.We hope that we could find new targets of tumor therapy.In the first part,wo focoused on GCTB.GCTB is a primary skeletal neoplasm.China is a high-risk group of GCTB.Usually,the remedial method of GCTB is surgery.However,in some typical cases,it's difficult to have a operation and the existing therapy ia not satisfied.We need new therapeutic methods.We showed differential expression of miRNA profiles between GCTB tissues and normal control by miRNA gene chip technology and predicted that miR-129 can bind to RANK.We then tranfected miR-129 into A549 by mimic and checked the protein level of RANK.But unfortunately,these preliminary work failed and we put forward a corresponding adjusting scheme.In the secondary part,we focoused on lung cancer.Lung cancer is the most prevalent tumor,with the highest morbidity and mortality over the wolrd.But unfortunately,the therapeutic methods of lung cancer did not get satisfied results.All of this remind us that more attention should be paid on the lung cancer and the new potential therapeutic targets are required.By consulting a source,we found that in most of lung cancer,the Ral was overexpression and Ral was selected as our target of the study.We up-regulated the level of RalA and RalB in A549,and check the fuction of RalA and RalB in A549 by CCK8,EdU and Transwell.We then used TargetScan to find some miRNAs that can bind to Ral.We then tranfected these miRNAs in A549 by mimic and checked the protein level of RalA and RalB in these transfected A549.We found that these A549 were weak at poliferation and invasion.We screened these miRNAs,and focused on miR-215.We use CCK8,Transwell and EdU recovery tests to prove that miR-215 can restrain lung caner by targeting the RalA and RalB.We provide theoretical basis for new target of lung cancer therapy.