Isolation Of Anti-cancer Composition From Bombys Batryticatus Extract And It Induced Apoptosis Pathway Against Mcf-7

Bombyx batryticatus,as an important medicinal resource insect,which was widely used in traditional Chinese medicine treatment.In this paper,the anti-cancer active components and mechanism of Bombyx batryticatus were studied by analyzing the activity of different extracts of Bombyx batryticatus alcohol extract and the induction of apoptosis of human breast cancer MCF-7.The main results are as follows:The results of high resolution mass spectrometry demonstrated that the ethanol extract of Bombyx batryticatus mainly contained protein,amino acid,ester,carbohydrate,flavonoids,nucleosides,organic acids,vitamins and terpenes.The extract of Bombyx batryticatus extract of petroleum ether mainly includes organic acids,nucleosides and amino acids.In the extraction phase of Bombyx batryticatus,the extract of petroleum extract of Bombyx batryticatus significantly inhibited the proliferation of MCF-7 cells and promoted the proliferation of normal cells BHK-21 to a certain extent.The ethyl acetate phase of alcohol extract of Bombyx batryticatus inhibited the proliferation of MCF-7 cells and promoted the proliferation of BHK-21 cells.The inhibitory effect of petroleum ether resuscitation on the viability of MCF-7 cells and the proliferation of BHK-21 cells were dependent on time and concentration.The morphology of MCF-7 cells changed with the increase of treatment time,and the number of cells was decreased and the shrinkage,deformation and rupture were observed.The extract of petroleum ether induced the apoptosis of MCF-7 cells,and the apoptotic rate was positively correlated with the concentration of petroleum ether extract of the ethanolic extract of Bombyx batryticatus and the concentration was 1.5 mg / mL,The total cell apoptosis rate was 19.42%,significantly higher than the control group.The cell cycle of MCF-7 cells was arrested in G1 phase and G2 phase,and the degree of retardation was positively correlated with the concentration.The concentration of ethanol extract of ethanolextract was 0.5 mg / mL,1.0 mg / mL,The sum of G1 phase and G2 phase was 68.58% and80.69% respectively,which were significantly higher than those of control.The gene expression on Bax,Bcl-2 and P21 in MCF-7 cells was affected by petroleum ether.both at mRNA level and at the level of protein expression.The concentration of petroleum ether extract was positively correlated with Bax and P21,positively correlated with Bax / Bcl-2,but negatively correlated with Bcl-2.The method of inducing apoptosis of human breast cancer cell line MCF-7 was achieved by up-regulating Bax,P21,down-regulation of Bcl-2 and increasing Bax / Bcl-2 ratio.