| Objective: In recent years,due to the improvement of the quality of life of residents,the incidence of obesity increased year by year,and its incidence is also showing a rising trend year by year,seriously reduce the health of the human body,thus,control obesity,reduce obesity is the current medical research The key lies.Serum TSH levels and obesity in the performance of a significant positive correlation between the use of acupuncture to lose weight is the focus of traditional Chinese medicine research in recent years to observe the acupuncture for obese rats serum TSH and adipocyte receptor effects,for the needle The mechanism of action of spiked prednisone in obese rats was studied.Methods: In this study,81 male Sprague-Dawley rats were randomly divided into control group and high-fat group.The control group was fed with normal diet and 58 rats in high fat group.And take the rats to harvest in each group.In the two groups of rats during feeding,the amount of feed and drinking water were not restricted.The body weight of the two groups of rats was measured every two weeks during feeding.Twenty percent of SD rats in the high fat group were overweight in the control group.For the obese group for continuous feeding for 44 days,and finally in accordance with the requirements of animal modeling,for those who do not meet the requirements of animal modeling removed,the remaining meet the requirements of rats in accordance with the random number table method,divided into specificacupoint acupuncture group,Acupuncture group,model control group,the number of rats in each group were 9.The rats in the normal control group and the model control group were treated with acupuncture at the request of the acupuncture group at the same time on the 45 th day,and no intervention was taken.According to the firm,The principle of the party solution for the specific meridian group take Zusanli,Zhongwan,Tianshu,Hong Leong,Yin Lingquan,Guan Yuan and other points,flat fill the line needle 1min,every10 min line needle 1,needle 30 min,Day 1,starting from the 45 th day of acupuncture treatment,to 75 d end,treatment time 30d).The rats were sacrificed by the method of aorta blood sampling after 8 hours of fasting treatment.The rats were sacrificed by the method of aorta blood sampling,and the rats were harvested at the time of taking the data at 0d,45 d,75d and so on.The rats were sacrificed and stored in a refrigerator.Pericardial fat,epididymal fat and Perirenal fat were removed and wighted.The cells were placed in a cryoprotective tube and stored in a liquid nitrogen tank in.The contents of TSH and LP(leptin)in the serum of obese rats were measured by enzyme-linked immunosorbent assay(ELISA).The expression of TSHR in the rat adipocytes was detected by immunohistochemical staining.The expression of TSH in the serum of obese rats was detected by enzyme-linked immunosorbent assay(Serum total cholesterol),TG(triglyceride)and HDL(high density lipoprotein)in obese rats were measured.Results:(1)The TSH content of each groups showed that the serum TSH level in the obese group was obviously higher than that in the control rats(P <0.001);(2)The TSHR(P<0.001).(3)Immunohistochemical results showed that the Perirenal adipocytes in obese group were obviously higher than that of the control rats(P<0.001).(3)The results of immunohistochemistry showed that the adipocytes Compared with the controlgroup,TSHR granules could be observed in the cytoplasm of fat cells in the two groups.The medium was expressed as the control group and the strong positive expression was obese.(4)The serum total cholesterol content and leptin of the two groups showed that the erum total cholesterol level and leptin in the obese rats was significantly higher than that in the control group(P<0.01);Conclusion: The level of TSH in the obese group was significantly higher than that of the control group.Acupuncture treatment could significantly reduce the weight of obese rats.The expression of TSHR in Perirenal adipose tissue was obviously higher than that in control rats.And it was found that acupuncture intervention could relieve the resistance of TSH in obese rats and promote the expression of TSHR in thyroid adipose tissue. |
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