Protective Effects Of Ajuga Decumbens Thunb On Lipopolysaccharide-induced Acute Lung Injury In Mice

Livestock and poultry respiratory diseases have a great impact on the current development of animal husbandry,which can cause huge economic losses to the animal husbandry because of its delay in the growth even death of livestock and poultry.As a medicinal plant,Ajuga decumben,whose main bioactive components is a class of iridoid glycosides such as 8-O-Acetylharpagide and Harpagide,is often used as an anti-inflammatory,antitussive and expectorant to treat chronic bromnchial inflammation.In this paper,we studied the anti-inflammatory effect of the extract of Ajuga decumben on airway inflammation and explored its therapeutic effect and mechanism on respiratory tract infection,and provided experimental basis for its clinical application.The results of this study are shown as follows:1.A week-long mice Acetylharpagide intragastric gavage test showed that Acetylharpagide attenuated the lung inflammation and reduced the IL-6,IL-1? and TNF-? content of serum in mice.At the same time,Acetylhar-pagide has no significant effect on the activity of CAT while it can increase the activity of GSH-PX and SOD and induce the content of MOD in mice serum.2.Two week-long mice Ajuga decumben decoction intragastric gavage test showed that Ajuga decumben decoction reduced the IL-6,IL-1? and T NF-? content of serum,apparently reduced the IL-6,IL-1? and TNF-? content of spleen and lung,and relieves the symptom of pulmonaryedema in mice.At the same time,Ajuga decumben decoction has no significant effect on the activity of CAT while it can increase the activity of GSH-Px and SOD in mice serum.3.Acetylharpagide intervention in alveolar macrophages of pig injured by LPS experiments showed that Acetylharpagide increased the activity of porcine alveolar macrophages,increased the genes expression level of IL-6 and NOS2 and reduced the genes expression level of IL-10 and Arg-1 in it.Among them,6.25?g/mL-25?g/mL Acetylharpagide has better anti-inflammatory effect.To summarize,in the LPS challenge mice test,Acetylharpagide attenuated the lung inflammation and reduced the IL-6,IL-1? and TNF-? content of serum in mice.Ajuga decumben decoction reduced the IL-6,IL-1? and TNF-? content of serum,apparently reduced the IL-6,IL-1? and TNF-? content of spleen and lung,and relieves the symptom of pulmonary edema in mice.It can be seen that when inflammation occurs in the lungs of mice,Ajuga decumben decoction inhibits its proinflammatory factors.Theref ore,the anti-inflammatory effect of it was achieved.In Acetylharpagide intervention in alveolar macrophages of pig injured by LPS experiments,Acetylharpagide increased the activity of porcine alveolar macrophages,increased the genes expression level of IL-6 and NOS2 and reduced the genes expression level of IL-10 and Arg-1 in it.In addition,the Ajuga decumben decoction can regulate the secretion of immune cytokines in mice,and then enhance the anti-inflammatory effect of it;Acetylharpagide can macrophages to M1/M2 phenotype polarization to regulate the downstream anti-inflammatory Target gene expression,and improve the anti-inflam matory level of cells.