The Investigation Of Morphological Features Of Cell Death And Tissue Remodeling After Fat Grafts

Objective To investigate the morphological features of adipocytes and tissue remodeling after fat grafts,and provide a theoretical basis of further improvement of fat graftsMethods The unilateral inguinal fat pad of C57BL/6J mice was autografted to the dorsa of the mice.Perilipin expression and morphological changes were investigated by immunohistochemistry staining,transmission electron microscopy and hematoxylin-eosin staining,respectively,in grafted tissues collected at posttransplantation days 0,1,3,5,7,14,and 30.Results Transmission electron microscopy analysis revealed that most adipocytes in grafts showed traits of cell death on day 3 after graft.Multilocular adipocytes with naive nuclei were observed as early as day 5 and a larger number of multilocular adipocytes were found on day 14.Hematoxylin-eosin staining found mang cyst and fibrosis after fat grafts.Perilipin immunostaining revealed that only some adipocytes located in the margin of grafts survived and the scope of viable adipocyte zonal are increased gradually from day 5 to day 30.Conclusions Most adipocytes in grafts died within 3 days after transplantation except those located in margin of grafts,and died cells will replace by new cells.It is suggested that enhance the outcome of graft should be obtained through promoting regeneration rather than improving survival of grafted adipose tissues.