Puerarin Derivative For The Effect Of Learning Memory And SOD And MDA In Brain On Cerebral Ischemia Reperfusion Dementia Model Mice

Vascular dementia is cognitive dysfunction syndrome which is due to all kinds of cerebrovascular disease.With the improvement of people's living standard and the society gradually entered the aging,the incidence of cerebrovascular disease is likewise increasing year by year.Thus,the incidence of vascular dementia is also increasing year by year.In clinic,the major performance of vascular dementia is the decline of learning and memory ability,mental deterioration,cognitive impairment and social adaptation ability is gradually weakened.Because vascular dementia has serious threat to the older people's quality of life and physical health,and brought a heavy burden to family and society.Therefore,treatment of vascular dementia is still a focus of current research.And compared to other types of dementia,vascular dementia can be prevented to some extent,and has better prognosis,has a broad prospect of treatment.VD can be prevented and controlled.Thus,exploring new drugs and seek effective treatment method,makes a very important social and medical significance.In clinical application,Puerarin is used primarily for the treatment of cardiovascular and cerebrovascular disease,and can protect and treat the cerebral ischemia-reperfusion injury.In order to further enhance its pharmacological effects.Drug researchers undertook transforming to the structure of puerarin,synthesized activated puerarin derivatives P1.Research of puerarin derivatives is still continuing.Therefore,we believe that in the near future,there will be more active and secure stronger puerarin derivatives.It is used for the clinical treatment of vascular dementia.In this thesis,vascular dementia model in mice was established by clamping of the both common carotid artery repeatedly.Detect puerarin derivative whether or not to get the effect of prevention and therapeutic of vascular dementia mice through the behavioral experiments and biochemical indicator detection experiments.Through behavioral experiments:water maze test and new object distinguish experiment to detect puerarin derivative P1 whether or not to have the effect of improving the ability of learning and memory for vascular dementia mice.By biochemical indicator detection experiments to detect puerarin derivative P1 whether or not to influence the SOD activity of hippocampus and MDA content of the cerebral cortex.To examine puerarin derivative P1 whether or not has an antioxidant effect.The experimental results can be shown that:compared with sham-operation group,the data of VD model group mice are significantly lower(P<0.05).In the water maze experiment,compared with the model group,advance to puerarin derivative P1(100mg/kg)dose group mice has been shortened the time of arrival in a safe area,but the mice have no significant difference with the model group(P>0.05).In new object distinguish experiment,1h and 24h of priority index and discrimination index of puerarin derivative P1(100mg/kg)dose group is higher than the model group,but the mice have no significant difference with the model group(P>0.05).In biochemical indicator detection experiments,puerarin derivative P1(100mg/kg)dose group and P1(25mg/kg)dose group mice have been improved the SOD activity in the hippocampus,and have been reduced the MDA content in the cerebral cortex,but the mice have no significant difference with the model group(P>0.05).To sum up,pretreatment puerarin derivative P1 for repeated occlusion bilateral common carotid artery induced dementia model mice have not significantly improved the ability of learning and memory.Puerarin derivative have no significant influence on the SOD activity of the hippocampus and MDA content of the cerebral cortex.Indicating that puerarin derivative P1 can not improve the antioxidant ability of vascular dementia mice.