The Extraction,Seperation And Structural Ananlysis Of Flavonoids From Valeriana Officinalis L.

Perennial herb Valeriana officinalis L.belongs to the family of Valerianace and genus of Valerian,of which Valeriana officinalis L.,Valeriana jatamansi Jones,Valeriana amurensis Smir.,Valeriana officinalis Linn.var.latifolia Miq.,etc are medicinal.Valeriana officinalis L.contains quite a few active ingredients,for examples,flavonoids,essential oil,alkaloids,saponins and polysaccharides.Flavonoids are a kind of secondary metabolites that exist widely in plant kingdom,having antioxidant,antibacterial,anti-tumor,liver protecting,radioresistance activity,etc.The research about the development of Valeriana officinalis L.have been reported mainly concentrated in the underground part,while there are few covers with reference to the overground part and flavonoids of Valeriana officinalis L.exist primarily in the branches and leaves.To make full use of Valeriana officinalis L.,the extraction,separation and structural analysis of flavonoids from Valeriana officinalis L.were studied.The main research results were showed as followings:Extracting flavonoids from Valeriana officinalis L.by microwave-assisted technology.The yield of flavonoids was used as index.Based on the single fact tests,the extraction process was optimized through response surface tests.The optimum extraction conditions were liquid-to-solid ratio 20 1 mL/g,ethanol concentration 47%,extraction temperature 66? and time 22 min.Under those conditions,the yield of the flavonoids was up to 7.90%.Extracting flavonoids from Valeriana officinalis L.by cellulase-ultrasonic technology.The yield of flavonoids was used as index.Combined with single factor tests and response surface experiments,the extraction conditions were optimized.The optimal process were cellulase concentration 1.9 U/mL,liquid-to-solid ratio 21.5:1 mL/g,ethanol concentration 48%,extraction temperature 45?,extraction time 61 min.Under the above conditions,the receiving rate of the flavonoids was up to 7.86%.Under the optimum extraction process,the yield of flavonoids of two kinds of process was closely,but it was found that the cellulase-ultrasonic technology was more convenient,simple,economical and needed lower temperature.Seprating flavonoids from Valeriana officinalis L..The absorption and desorption rate of resins to flavonoids were used as main indexs.The optimum resin was chosen from 6 kinds of resins by the static absorption-desorption tests and it was discovered that polyamide resin was the best to seprate flavonoids from Valeriana officinalis L.The sample and elution conditions were optimized by the dynamic absorption-desorption experiments.The results showed that the optimum sample conditions were concentration 2.0 mg/mL,velocity 1.5 mL/min,amount 20 mL and pH value 5.5,the optimal elution conditions were ethanol concentration 70%,velocity 2.0 mL/min and amount 70 mL.Under those conditions,the purity of flavonoids was from 17.58%up to 79.17%.The structure analysis of flavonoids from Valeriana officinalis L..Flavonoids from Valeriana officinalis L.having been separated were further isolated and depurated by macroporous resin HPD600 and Sephadex LH20 through gradient elution and four pure compounds were gained.The above four compounds were preliminarily identificated that were respectively rutin,quercetin,kaempferia galanga phenol and celery by UV,IR and HPLC-MS.