Study On The Action Part Of Orientation Change Of Jiao Ai Decoction Evolved Into Si Wu Decoction

Jiao ai decoction is a classical and practical formula that firstly recorded in'Jin-Gui-Yao-Lue' by a physician Zhongjing Zhang.lt is composed of seven herbs including Chuanxiong rhizoma,Asini corii colla,Glycyrrhizae radix et rhizoma,Artemisiae argyi folium,Angelicae sinensis radix,Paeoniae radix alba and Rehmanniae radix.According to the clinical indications woman rushed any deficiency,the collapse in leakage,water too much,more than the shower drain,or with postpartum blood continuously,or pregnancy blood and stomach pain.Si wu decoction is firstly recorded in 'Xian-Shou-Li-Shang-Xu-Duan-Fang',by three kinds of medicines of Jiaoai Decoction minus donkey hide gelatin,mugwort leaf,liquorice,Rehmannia glutinosa is easily prepared rhizome of rehmannia,and increased Ligusticum chuanxiong and Angelica two ingredients proportion is evolved.Jiaoai Decoction derived Siwu Decoction is by refining the original,highlighting the invigorate the circulation of blood of the efficacy of drugs combination proportion;the second is to increase the proportion of Radix Angelica sinensis and Rhizoma Chuanxiong by adjusting the dose,the prescription effect to nourishing and blood direction of expansion.The earlier stage of the project focused on the research of Jiaoai soup derived Siwu Decoction effect changes in orientation,and of Jiaoai soup evolved into Siwu Decoction effect changes in the orientation of material basis and mechanism of little.Therefore the Jiaoai Decoction and Siwu Decoction and different solvent extracts as the research object,with the aid of the acute blood stasis model,uterine bleeding,the blood deficiency model screening the most effective blood effect of Siwu Decoction,the most effective hemostasis and blood enriching parts of jiao ai tang;Through a comparative study on UPLC-Q/TOF-MS of Jiaoai decoction,Siwu Decoction and its different solvent extraction chemical constituents were identified,so as to provide the basis for material basis to clarify jiaoaitang derived Siwu Decoction for changing orientation effect.This tensis mainly includes the following parts:Part one:Literature reviewThrough the review of the related literature at home and abroad,mainly from two aspects of the chemical components and pharmacological action of Jiaoai Decoction and Siwu Decoction and related ingredients were reviewed,clearly understand the research status of Jiaoai Decoction and Siwu Decoction,formulate feasible experimental schemes for the artical,to clarify the substance basis of jiaoaitang derived Siwu Decoction for changing orientation effect.Part two:Screening of effective parts of Siwu Decoction in acute blood stasis model rats promoting blood circulationAfter si wu decoction by boiling water,the system solvent extraction method is adopted four solvent extracted parts respectively.Ice water bath and subcutaneous injection of adrenaline hydrochloride were both used to establish the acute blood stasis rat model.The whole blood viscosity,plasma viscosity,blood coagulation,blood platelet aggregation rate,plasma TXB2,6-keto-PGFla and uterine pathological sections of the rats were detected.Compared with the model group,ethyl acetate parts can significantly reduce the acute blood stasis model rats with the shear rate of whole blood viscosity,plasma viscosity(P<0.01),Significantly extend TT,PT,APTT(P<0.01),significantly reduced the content of fibrinogen(FIB)(P<0.01),for the platelet aggregation has obvious inhibitory effect(P<0.01),can significantly reduce the content of plasma TXB2(P<0.01),elevate the content of plasma 6-keto-PGF1a(P<0.01);Petroleum ether group can decrease the whole blood viscosity under high shear rate(P<0.05),reduce TXB2 content,increase the content of 6-keto-PGFla(P<0.01);N-butyl alcohol parts and water parts can obviously reduce TXB2 and increase 6-keto-PGF1a.(P<0.01).Prompt,Ethyl acetate part is the most effective part of si wu decoction on removing blood stasis.Part three:Screening effective parts of Jiao ai Decoction on uterine hemorrhage rat model of hemostatic effectAfter jiao ai decoction by boiling water,the system solvent extraction method is adopted four solvent extracted parts respectively.Mifepristone and Misoprostol were given to early-pregnancy rats orally to establish the uterine hemorrhage model rat,estradiol(E2),progesterone(P),luteinizing hormone(LH),follicle stimulation hormone(FSH),coagulation function,blood platelet system,uterine bleeding time(UBT),uterine bleeding(UBQ),uterine gross and histology figure were tested.The results show,compared with the model group,ethyl acetate parts can significantly increase E2(P<0.01),P(P<0.01),LH(P<0.01),FSH(P<0.01),significantly shorten TT(P<0.01),PT(P<0.01),APTT(P<0.05),increased FIB(P<0.01)and the contents of TXB2 increased(P<0.01),decreased the content of 6-keto-PGFla(P<0.01),regulating the balance of TXB2/6-keto-PGFla(P<0.01),significantly shorten UBT(P<0.05),reduced UBQ(P<0.01);N-butyl alcohol group can significantly increase LH,6-keto-PGFla,regulating the balance of TXB2/6-keto-PGFla(P<0.01);water group can significantly increase E2,FSH,FIB(P<0.05),shortened TT(P<0.05),increased TXB2(P<0.01),reduced 6-keto-PGFla(P<0.05),regulating the balance of TXB2/6-keto-PGFla(P<0.01),shortened UBT(P<0.05),reduced UBQ(P<0.05).Prompt,Ethyl acetate part is the most effective part of jiao ai decoction on blood protection effects.Part four:Screening effective parts of Jiao ai Decoction on blood enriching function in blood deficiency model ratsAfter jiao ai decoction by boiling water,the system solvent extraction method is adopted four solvent extracted parts respectively.Take every day on the fundus venous plexus put blood 5 mL·kg-1,12 d continuous bleeding,to establish the rat model of blood deficiency;Modeling and animals in each group were ig given Drug for trial.peripheral hemogram and immune organ index and leukocyte mediated IL 2(IL-2)and erythropoietin(EPO)levels and erythrocyte membrane energy metabolism enzyme activity were tested.The results show,compared with the model group,N-butyl alcohol group can significantly increase HCT,RBC,HGB,PLT(P<0.01,P<0.05);reduce the spleen index and the content of EPO(P<0.01,P<0.05);increased thymus index,the content of IL-2(P<0.01),elevated erythrocyte Ca2+,Mg2+-ATP enzyme and Na+,K+-ATP enzyme activity(P<0.01,P<0.05).Petroleum ether group could obviously increase the content of PLT and IL-2(P<0.01),decreased the content of EPO and the spleen index(P<0.01,P<0.05).The ethyl acetate fractions can obviously reduce the spleen index and the content of EPO(P<0.01),increased Ca2+,Mg2+-ATP enzyme activity in erythrocytes(P<0.05).Water group can significantly high PLT(P<0.05),decreased the content of EPO(P<0.01).Prompt,N-butanol extract was the most effective part of Jiaoai Decoction for enriching the blood.Part five:A comparative study on the hemostatic efficacy of the different proportions of the ethyl acetate part and the different proportion combination of the parts of N-butanol part in the rat model of uterine bleedingJiaoai Decoction Herbal Pieces decocted with water after the system solvent extraction method of Jiaoai soup of ethyl acetate extract and Jiaoai Tang n-butanol extract,Jiaoai soup of ethyl acetate extract and Jiaoai soup n-butanol according to different weight than combined with 0.5%CMC Na solution formulated as the solution for the test items.Mifepristone and Misoprostol were given to early-pregnancy rats orally to establish the uterine hemorrhage model rat,estradiol(E2),progesterone(P),coagulation function,blood platelet system,uterine bleeding time(UBT),uterine bleeding(UBQ)were tested.The results show,compared with the model group,Ethyl acetate part:N butyl alcohol 1:3 group can significantly shorten TT,PT,APTT,increased FIB significantly shorten UBT,reduced UBQ,the contents of TXB2 increased,decreased the content of 6-keto-PGFla,regulating the balance of TXB2/6-keto-PGFla,can significantly increase E2,P.Prompt,Ethyl acetate part::N butyl alcohol 1:3 group compared with other drug groups,the bleeding symptoms of rats were significantly alleviated,it is the best formula of the hemostatic effect of the rats in the model rats of the uterine bleeding model.Part six:Jiaoai Decoction,Siwu Decoction and its different solvent extraction parts of the body's specific immune and non-specific immune function effect in vitro1.Jiaoai Decoction,Siwu Decoction and its different solvent extraction parts of the body's non-specific immune function effect in vitroCultured in vitro of rat peritoneal macrophages as the object of study examines the Jiaoai Decoction and Siwu Decoction and its different solvent extracts of rat peritoneal macrophage phagocytosis,proliferation and in vitro induced effects of cytokines.The results show,Jiaoai Decoction and Siwu Decoction and its different solvent extraction parts in the safe dose range had different degrees of enhancement on non-specific immune function.Jiaoai Decoction compared with Siwu Decoction,the Jiaoai decoction has better promote macrophage phagocytosis of neutral red(NR)and promote the proliferation of macrophages and enhance the cytokine IL-12 and TNF-a levels so as to enhance the role of non-specific immune function,the non specificity immune function of the body of the rubber is the best in the rubber.2.Jiaoai Decoction,Siwu Decoction and its different solvent extraction parts of the body's specific immune function effect in vitroIn vitro cultured mouse spleen lymphocytes as the object of study examines the Jiaoai Decoction and Siwu Decoction and its different solvent extraction parts of mouse spleen lymphocyte proliferation in vitro,ConA and LPS induced proliferation of spleen lymphocytes,cytokine IL-2 and TNF-a in effect.The results show,Jiaoai Decoction and Siwu Decoction can enhances the proliferation of spleen lymphocyte of mice and with mitogen ConA and LPS synergistically promote the proliferation of spleen lymphocyte of mice and enhance cell factor IL-2,TNF content and Jiaoai decoction is obviously better than that of Siwu Decoction,In the different solvent extracts of different solvents,the improvement of the above mentioned indexes was the most obvious in the gel.Part seven:UPLC/Q-TOF-MS analysis of qualitative chemical component extraction of Jiaoai decoction,Siwu Decoction and its different solventUsing AB SCIEX Triple TOFTM5600 high resolution mass spectrometry,an ACQUITY UPLC(?)HSST3C18 chromatographic column(2.1 x 50 mm 1.8 ?n),with 0.1%formic acid in water and acetonitrile as mobile by gradient elution.ESI ion source collects data in negative ion mode,and data analysis is carried out with Peak View SoftwareTM Jiaoai Decoction identification of the Communist Party of China out of 73 chemical composition,Jiaoai Decoction of petroleum ether fraction were identified 36,Jiaoai Decoction of ethyl acetate were identified 58 chemical composition,the Jiaoai soup is butanol were identified 65 chemical composition,Jiaoai soup site identified a total of 58 chemical composition,Siwu Decoction were identified 48 chemical constituents,Siwu Decoction of petroleum ether fraction were identified 25 chemical composition,Siwu Decoction ethyl acetate fraction identified a total of 39 chemical composition,Siwu Decoction is butanol were identified 43 chemical composition,Siwu Decoction parts were identified and 32 chemical constituents.Part eight:In the early stage of the(2),(3),(5),(7)based on the work,through the SPSS analysis software of bivariate correlation analysis and correlated blood tonic effect.The main chemical compositionofpaeoniflorin,oxypaeoni:florin,paeoniflorinsulfonate,benzoyl-oxypaeoniflorin,Muda npiosideE,schaftoside,Liquiritin,Isoliquiritin,Glycycoumarin,Glyasperins K,8-epiloganic acid,Uridine,Ligustilide,apigenin,rhamnetin;And invigorate the function was significantly related to chemical composition are mainly Vanillic acid,caffeic acid,4-hydroxy-3-butylphthalide,gallic acid,4-hydroxy-3-butyl phthalide isomer,Ethyl gallate,trans-frulic acid,senkyunolide J;Correlated with hemostatic effect.The chemical composition of stachyose,gallic acid ethyl ester,Paeonolum,Glycyrrhizin,Phthalic acid dibutyl,3,6-two-phenyl ketone.The apigenin can play a role of blood,invigorate the circulation at the same time,gallic acid ethyl ester can play a role of activating blood,stop bleeding at the same time.