Preliminary Discussion Huaiyang Medicago Polymorpha(seeding Grass)Simple Obesity And Lipid Metabolism In Rats And Its Molecular Mechanism

Objective:Establish simple obesity rat model,discuss the role of lipid-lowering diet Huaiyang Medicago polymorpha(grass seedlings)to high-fat diet induced simple obesity rats,and to explore the molecular mechanisms of lipid metabolism.Methods:Body weight 200±20 SPF male SD rats were 71,divided i nto control group(10)and model group(60),respectively,to the norma 1 diet and high-fat diet for nine weeks.The successful model rats were randomly divided into model group,seeding grass high dose group,mid dle dose group seeding grass,grass seedlings low-dose group and the p ositive control group,continued feeding with high fat diet control grou p still to be ordinary feed.Seeding grass high,medium and low dose gr oup and the positive control group were fed grass seedlings lyophilized powder suspension 2g/(kg·d),1 g/(kg · d),0.5g/(kg · d)and Orlistat aqueous 35 mg/(kg · d),for 6 weeks.Weekly time measured weight.After the last dose fasting 12h.measurement of body weight,b ody length,abdominal circumference,liver weight,kidney weight,perire nal fat weight and epididymal fat weight:calculated lee's index;blood serum,automatic biochemical analyzer TC,LDL-C.TG and HDL-C lev els;Liver HE staining and microscopic morphological observation;RT-PC R detection of liver tissue LCAT,CYP7 A1 HMGCR mRNA expression levels and;Western blot detection of liver PPAR ?,CYP7 A1,expressi on of LCAT and HMGCR;immunohistochemistry liver LXR,ABCA1,SR-BI protein expression.Results:After modeling nine weeks,the average weight of model group was significantly higher than the control group,the average body weight(P<0.01).Six weeks after treatment,compared with the control group,the model group rats body weight.body length,abdominal circumference.Lee's index.liver weight,kidney weight index and organ fat wet weight increased significantly(P<0.01,P<0.05);serum TC,TG and LDL-C significantly increased,HDL-C decreased significantly(P<0.01).Liver CYP7 Al,LCAT,LXR,ABCA1 and significantly up-regulated expression of SR-BI(P<0.01,P<0.05).Compared with the model group,the body weight of each rat administration group,liver weight,kidney weight,organ fat index and wet weight decreased significantly(P<0.01);seeding grass high and low dose group and positive control group significantly reduced abdominal circumference(P<0.05);seeding grass high dose group Lee's index was significantly reduced(P<0.05);seeding grass high dose group and positive control group TC,TG and LDL-C decreased,HDL-C increased significantly(P<0.01),TC grass seedlings dose group,LDL-C lowering,HDL-C increased(P<0.01,P<0.05);liver PPAR ? seeding grass high dose group and the positive control group,HMGCR protein was significantly increased(P<0.05),LXR,ABCA1 and SR-BI protein expression was significantly reduced.Compared with the positive control group,the liver PPAR ?,CYP7 A1,LCAT protein expression was significantly lower(P<0.01,P<0.05),HMGCR and LXR protein expression significantly increased(P<0.05).Control group of liver cell structure clear,uniform cytoplasm.Model control group of liver cell disorder,intracytoplasmic vacuoles round fat droplets.Seeding grass high,medium and low dose group and the positive control group of liver cells arranged in neat,liver cells than in normal group compared with enlargement,there is a small amount of fat droplets vacuole formation.Conclusion:Fat diet obese rat model can be established,seeding grass can reduce body weight and body fat in obese rats,decreased scrum T C,TG and LDL-C.increased HDL-C levels.The mechanism may be reg ulated by the liver PPAR ?.CYP7 A1,LCAT protein expression,promo te cholesterol decomposition,transport and excretion,inhibition of chole sterol synthesis.play a role in lipid-lowering die-t.