Alfalfa Extract Of Saponins Induced Cell Death In A549 Lung Cancer Cells

Saponins(saponins)is a kind of structure more complex nucleoside compounds,widely exist in nature.Is one of the important biological active ingredients,many of the traditional Chinese medicine(TCM)experimental proof.Saponins have fall blood sugar blood fat,antiviral,immunity and inhibit tumor effect,has become a hot topic of research at home and abroad.The study found that saponins in a wide variety of tumor cells play a role of anti-tumor.A variety of saponins can make the cell cycle arrest,inhibiting tumor cell proliferation,promote cell apoptosis.And saponins with traditional cancer treatments also has the very good effect.Many natural Oleanane saponins has a significant anti-tumor activity,research shows that contains 3-sugar chain,28-carboxyl's pier fruit alkanes type saponins have good inhibitory activity on tumor.As the research object,based on alfalfa saponin extract alfalfa saponins extract found in inducing apoptosis of A549 cells.In order to study the mechanism of action of alfalfa saponins extract induced cell death in A549 lung canaer cells,we use different concentration of alfalfa saponins extract processing human lung adenocarcinoma cells(A549),through the 2-(2-4-nitro phenyl methoxy-)-3-(4-nitro phenyl)-5-(2,4-disulfonic acid benzene)-2 h-tetrazolium monosodium salt(CCK 8),acridine orange(AO)staining,Hoechst33234 and Propyl iodide organism(PI)double dye,detection of mitochondrial membrane potential by JC-1;Western-bloting test to study the induction of A549 cell death.In order to study the mechanism of action of alfalfa saponins extract induced cell death in A549 lung canaer cells,we use different concentration of alfalfa saponins extract processing human lung adenocarcinoma cells(A549),through the 2-(2-4-nitro phenyl methoxy-)3-(4-nitro phenyl)-5-(2,4-disulfonic acid benzene)-2 h-tetrazolium monosodium salt(CCK 8),acridine orange(AO)staining,Hoechst33342 and Propyl iodide organism(PI)double dye,detection ofmitochondrial membrane potentialby JC-1;Western-bloting test to study the induction of A549 cell death.Western bloting detection analysis of cytochrome C,Caspase-3,Caspase-8,Caspase-9,the amount of the expression of Bax and Bid,Bak results show that the concentration in 50 ?g/m L,100 ?g/m L,200 ?g/m L with the increase of concentration of expressing quantity is less,the 300 mu g/ml,400 ?g/m L expression quantity as concentration rises.