Role Of Scutellaria Barbata Extract On Proliferation And Apoptosis Of Lung Cancer A549 Cells And Its Relationship With Endoplasmic Reticulum Stress

Objective : To explore the relationship between the effect of Scutellaria barbata extract on the proliferation and apoptosis of lung cancer A549 cells and endoplasmic reticulum stress.Methods:Lung cancer A549 cells cultured in vitro were used.1.To determine the effect of Scutellaria barbata extract on the proliferation and apoptosis of Lung cancer A549 cells,lung cancer A549 cells were treated with different concentrations of Scutellaria barbata extract,including group A(negative control group),group B(10mg / L of Scutellaria barbata extract),group C(20mg / L of Scutellaria barbata extract)and group D(40mg / L of Scutellaria barbata extract).After treatment for indicated time(24,48,72 h),the cell proliferation was detected by tetramethylazozol blue(MTT)colorimetry;cell apoptosis was analyzed by flow cytometry;western blot was used to detect the expression of the endoplasmic reticulum stress marker proteins such as GRP78 / Bip,CCAAT / enhancer binding protein homologous protein(CHOP)and cysteine aspartic protease-12(Caspase-12).2.To explore the relationship between the effect of Scutellaria barbata extract on the proliferation and apoptosis of lung cancer A549 cells and endoplasmic reticulum stress,lung cancer A549 cells were divided into 3 groups including group A(negative control group),group B(SBE40mg / L,which serve as the optimal concentration)and group C(SBE40mg / L plus 4-PBA inhibitor).After treatment for indicated time(24,48,72 h),the cell proliferation was detected by MTT;cell apoptosis was analyzed by flow cytometry;western blot was used to detect the expression of GRP78 / Bip,CHOP and Caspase-12.Results:1.Effects of Scutellaria barbata extract with different concentrations on the proliferation and apoptosis of lung cancer A549 cells.(1)Compared to the negative control group,the treatment of lung cancer A549 cells with different concentrations(10mg / L,20 mg / L,40 mg / L)of Scutellaria barbata extract for 24 to 72 h can significantly inhibit their proliferation with a time-dose dependent manner.The difference is statistically significant(P <0.05).(2)Compared to the negative control group,the apoptosis rate of lung cancer A549 cells treated with Scutellaria barbata extract at different concentrations(10mg / L,20 mg / L,40 mg / L)for 48 hours was significantly higher.The difference was statistically significant(P <0.05).(3)Compared to the negative control group,the protein level of GRP78 / Bip,CHOP and Caspase-12 in the Scutellaria barbata extract treatment group were significantly increased,and the differences were statistically significant(P <0.05).2.To explore the relationship between the effect of Scutellaria barbata extract on the proliferation and apoptosis of lung cancer A549 cells and endoplasmic reticulum stress.Through treatment of ERS inhibitor(4-PBA),the proliferation inhibition rate,apoptosis rate and the protein level of GRP78 / Bip,CHOP and Caspase-12 of lung cancer A549 cells were significantly reduced.The difference was statistically significant(P <0.05).Conclusion:1.Scutellaria barbata extract can inhibit the proliferation of lung cancer A549 cells and promote apoptosis.2.Scutellaria barbata extract may inhibit the proliferation and promote apoptosis of lung cancer A549 cells through regulating endoplasmic reticulum stress.