The Depressant Effect Of Dau To BxPC-3 Cell Line Of Pancreatic Cancer Based On Erk Signaling Pathways

Purpose:The purpose of the present study is to observe the anti-proliferation of dauricine(Dau)on pancreatic cancer BxPC-3 cells.To observe the effect of Dau on the key factors,explore the possible mechanism of ERK signaling pathway.Methods:Pancreatic cancer BxPC-3 cells were cultured in vitro.The trial included:the control group(normal culture medium),Dau high dose group(48μg/ml),Dau low dose group(24μg/ml),5-FU group(50μg/ml)1.This paper applied trpan blue staining method to test the growth cycle of the BxPC-3 cells;2.Cell wound scratch assay was used to determine the migration ability of the BxPC-3 cells;3.Methyl thiazolyl tetrazolium(MTT)experiment was employed to measure the proliferative inhibition of the BxPC-3 cells;4.Flow cytometry(FCM)was used to measure the apoptpsis of the BxPC-3 cells;5.Quantitative Real-time PCR was adopted to analysis the expression of Raf mRNA and MEK1 mRNA;6.Western blot was employed to test the expression of Raf protein and MEK1 protein.Results:1.Dau could significantly restrain the cell proliferation of pancreatic cancer BxPC-3 cells.2.Dau could remarkably reduce the migration ability of the BxPC-3 cells(P<0.01).3.D48 group,D24 group and 5-FU group,the cell proliferation inhibition rates were 70.81%,44.62%and 58.50%.4.D48 group,D24 group and 5-FU group could increase the cell apoptosis rate,compared to the control group,there were significant differences(P<0.01).5.Dau could reduced the expression of Raf mRNA and MEK1 mRNA(P<0.01)6.Dau could reduced the expression of Raf protein and MEK1 protein(P<0.05 orP<0.01).Conclusion:1.Dau could remarkably restrain the cell proliferation and migration ability of pancreatic cancer BxPC-3 cells.2.Dau could induce cell apoptosis3.Dau could down regulate the expression of Raf,MEK1 mRNA and protein on ERK signaling pathway.4.Our data indicates that the anti-proliferation effect of Dau is associated with inhibition of ERK signal pathway.