Screening And Evaluating Of Composition Which Have Es Trogen-like Activities In Xiaoyao Powder

Objective:By studying the effects of ten active ingredients in Xiaoyao pow der on the proliferation of mammary cancer cell MCF-7,in vitro to dis cuss its potential mechanism and preliminary clarify the mechanism of active ingredients in Xiaoyao powder for estrogen-like activities,and to provide theoretical basis for rational use of Xiaoyao powder in treating Perimenopausal syndrome.Methods:The ER-positive cell MCF-7 was treated with ten active ingreden ts in Xiaoyao powder which include Umbelliferone?Ferulic acid?L icoflavone?Liquiritigenin?Glycyrrhizin?Saikogenin-d?Quercetin?Kaempferol?Isorhmnetin?Glycyrrhetinic and estrogen as well as ICI 1 82780.MTT test was used to detect the cell proliferation,Western Blot and RT-PCR were respectively adopted to test E??ER? protein and mRNA expression level.Results:Umbelliferone(100?mol/L)?Ferulic acid(100?mol/L)?Saikogeni n-d(100?mol/L)?Licoflavone(100?mol/L?10?mol/L?1?mol/L?10-1?mol/L)?Liquiritigenin(10?mol/L?1?gmol/L?10-1?mol/L)?Glycyrrhiz in(100?mol/L?10?mol/L)?Quercetin(100?mol/L?10?mol/L)can signi ficantly inhibit the MCF-7 cell proliferation(P<0.05),which showed significant cytotoxity on human melanoma and didn't fit for the next research.Quercetin(1 0-2?mol/L?10-3?mol/L)?Kaempferol(0-2?mol/L?10-3?mol/L)?Isorhmnetin(10-3?mol/L)?Glycyrrhetinic(100?mol/L??mol/L?10-2?mol/L)can significantly promote the MCF-7 cells pr oliferation(P<0.05);The Western Blot result showed that 1?mol/L Qu ercetin,10?mol/LKaempferol?Glycyrrhetinic,100?mol/L Isorhmnetin can raise ER??ER? protein level(P<0.05);The RT-PCR result showed that 1?mol/LQuercetin,10?mol/LKaempferol?Glycyrrhetinic,100?mo 1/L Isorhmnetinl can raise ER??ER? mRNA level(P<0.05);The above effects can be blocked by adding estrogen receptor antagonisrn ICI182780.Conclusion:Quercetin?Kaempferol?Isorhmnetin?Glycyrrhetinic are the ac tive ingredients in Xiaoyao powder for estrogen-like activities.they c an promote ER-positive MCF-7 proliferation,raise ER??ER? protein and mRNA level.It is speculated that they have the estrogen-like acti vities through the estrogen response pathway.In addition,Umbellifero ne?Ferulicacid?Saikogenin-d?Licoflavone?Liquiritigenin?Glycyrrhi zin can significantly inhibit the MCF-7 cell proliferation for anti-est rogen activity.But it has yet to the further study of its potential mech anism.