| ObjectiveWe aimed to explore the mechanism of let-7a in the development of gastric cancer by testing the expression of let-7a in gastric cancer tissues,adjacent para-cancerous tissues and distal normal gastric tissues;Another aim was to explore the regulation mechanism of chinese herbal compound Yangzheng Sanjie Decoction(YZSJD)on gastric cancer through observing the influence of YZSJD on proliferation,apoptosis and the expression of let-7a in gastric cancer cells.MethodsClinical research:11 cases of gastric cancer who received radical total gastrectomy were collected.The specimens of the patients' tumor tissues,adjacent para-cancerous tissues and distal normal gastric tissues were collected immediately.All the specimens were fixed in 4%formalin,embedded with paraffin and tested the expression of let-7a by in situ hybridization.Experimental research:The AGS cell and HS-746T cell were selected in vitro as the model cell lines of gastric cancer.Both of the two cell lines were cultured with 10%of YZSJT-containing rat serum(YZSJT group)and non-medicated rat serum(control group)respectively,then the cell proliferation was measured using Cell Counting Kit-8 assay,the cell apoptotic were detected by flow cytometry of Annexin V-FITC/PI staining,and the influence on the expression of let-7a was analyzed by real time PCR.Results1.In the distal normal gastric tissues,the adjacent para-cancerous tissues and the gastric cancer tissues,the expression of let-7a were(165.45±77.64)%,(83.64±39.31)%and(41.36±39.06)%,and the difference were statistically significant among the three groups(P<0.05).2.After intervened with YZSJD-containing rat serum,the proliferation of the AGS cell and the HS-746T cell were significantly lower than the control group(P<0.01),and the proliferation inhibition rate were 22.4%and 19.5%.3.Compared with the control group,after intervened with YZSJD-containing rat serum,the total apoptosis rate of the AGS cell and the HS-746T cell showed no significant difference(P>0.05).4.The relative expression of let-7a in the AGS cell was(1.60±0.08)after dealed with YZSJD-containing rat serum,and which was obviously up-regulated compared with the control group(P<0.01).Conclusion1.The expression of let-7a in the normal tissues,the adjacent cancerous para-tissues and the gastric cancer tissues were gradually decreased.It suggested that let-7a might act as a tumor suppressor involved in the development of gastric cancer.2.YZSJD-containing rat serum could significantly inhibit the prolife-ration of the AGS cell and the HS-746T cell lines.3.YZSJD-containing rat serum could up-regulate the expression of let-7a in the AGS cell.It suggested that regulating the expression of let-7a in gastric cancer might be one of the mechanisms of YZSJD action. |
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