Association Of Genetic Variation Of LncRNA HOTAIR Signaling Pathway With Abdominal Obesity And Fat Distribution In Children And Adolescents

Objectives(1)To understand the lifestyle and behavioral factors that affect abdominal obesity and fat distribution in children and adolescents.(2)To investigate the effect of genetic variants of the LncRNA HOTAIR regulatory pathway on abdominal obesity and fat distribution through association analysis.(3)To evaluate the influence of genetic variants,genetic variants-life behavioral factors interaction on abdominal obesity and fat distribution.MethodsBased on the regular physical examination platform for students of the Health Promotion Center for Primary and Secondary School Students of Guangzhou,this study randomly selected 4,300 students from 39 urban primary and secondary schools,grade 2 of primary school,grade 1 of junior and senior high school in Guangzhou for investigation and physical examination from June to November 2016.Calculated WHtR and WHR.According to two criteria(WHtR criteria,WHR criteria),divided into abdominal obesity group and nonabdominal obesity group.The WHtR and the WHR normalized by gender and grade are as indicators of fat distribution.Bioinformatics techniques were used to predict LncRNA HOTAIR regulatory pathways and screen potential functional genetic variants.TaqMan real-time quantitative PCR was used to detect the genotypes.Logistic regression and multiple linear regression were used to analyze the association between lifestyle behaviors,genetic variants and abdominal obesity and fat distribution.The generalized multifactorial dimension reduction method was used to analyze the effects of interactions between genetic variants,genetic variants-life behavioral factors on abdominal obesity and fat distribution.Results(1)In the abdominal obesity group and the non-abdominal obesity group selected by the WHtR and WHR criterias,good appetite increases the risk of abdominal obesity(WHtR criteria: OR=2.23,95%CI=1.79-2.80;WHR criteria: OR=1.40,95%CI=1.06-1.86).After adjusted for gender and grade,multiple linear regression models showed that a good appetite could increase the WHtR by 0.0166(P<0.0001)and the standardized WHR by 0.1820(P<0.0001).(2)Among the abdominal obesity patients screened by the WHtR criteria,the other group of CDKN1 A diplotype are associated with WHtR.Compared with the ACC/CAT,the other group can reduce the WhtR by 0.0119(P=0.0448).In abdominal obesity patients screened by the WHR criteria,ACC/CCC reduced the standardized WHR by 0.5251(P=0.0172).(3)GMDR results showed that there was a second-order interaction between rs11202592 and rs762624(P=0.041).At the same time,there was a third-order interaction between HOTAIR,CDKN1 A diplotype and appetite(P<0.001).Among the abdominal obesity patients screened by the WHtR criteria,GMDR found second-order model of rs11202592 and rs1059234(P=0.021),fourth-order model of rs920778,rs1801270,appetite and the weekend screening time(P=0.027),fifth-order model of taste,appetite,weekend screening time,HOTAIR and the CDKN1 A diplotype(P=0.013)had interactions that affected WHtR.Among the abdominal obesity patients screened by the WHR criteria,GMDR found fourth-order model of rs920778,rs11202592,rs1059234,and rs762624(P=0.002),and HOTAIR and CDKN1 A dimorphic second-order model(P=0.016)had interactions that affected standardization WHR.No interactions between genetic variants,genetic variants and lifestyle behavior were found on abdominal obesity and WHtR.Conclusions(1)Good appetite is a risk factor for abdominal obesity and fat distribution.(2)Compared with the CDKN1 A ACC/CAT,WHR levels were lower in patients with abdominal obesity who carried the ACC/CCC.(3)The interactions of the genetic variants of the LncRNA HOTAIR pathway and genetic variants and life behavior factors affected the severity of abdominal obesity.