Expression And Significance Of MiR-27a-3p In Lung Adenocarcinoma

ObjectiveDue to high morbidity and mortality,malignant behaviors and lack of effective treatment,lung cancer is deemed to the leading cause of cancer-related deaths,worldwide.Investigations on driving forces behind the outgrowth capacity of lung cancer cells are urgently needed for understanding of lung cancer pathogenesis and valuable for novel therapeutic explorations.In previous studies,many mi RNAs have been aberrantly altered in NSCLC and contribute to the development and progression of NSCLC.They function as oncogenes or tumor suppressors via targeting different targets.This subject takes lung adenocarcinoma as the research object.To investigate the expression of miR-27a-3p in tumor tissue of patients with lung adenocarcinoma and explore the effect of miRNA-27a-3p expression on invasion and migration of lung adenocarcinoma cells.Methods1.The 21 operation samples including normal(5 cm away from the edge of tumor)and tumor tissue were taken from the lung adenocarcinoma patients in the Affiliated Hospital of Qingdao University as research object,all patients had not been treated by radiotherapy nor chemotherapy,post operation pathogical result indicated lung adenocarcinoma.These samples were transferred to EP tubes as soon as possible,and then transferred to-80 ? ultra low temperature refrigerator for long time preservation.2.The tumor tissues of 21 lung adenocarcinoma patients and corresponding adjacent normal tissues were collected for this study and the differences of mi R-27a-3p expression levels in tumor tissues and adjacent tissues were detected by real-time fluorescence quantitative PCR.3.The expression of miR-27a-3p in A549 cells was overexpressed and silenced by transfecting miR-27a-3p mimics and miR-27a-3p inhibitor.4.The proliferation ability of A549 cells after transfecting was detected by MTT assay to verify the effect of mi R-27a-3p expression on proliferation ability of lung adenocarcinoma cells.5.The migration ability of A549 cells after transfecting was detected by cell scratch assay to verify the effect of mi R-27a-3p expression on migration ability of lung adenocarcinoma cells.Results1.The expression levels of miR-27a-3p in tumor tissues of lung adenocarcinoma patients were significantly higher than adjacent non-cancerous tissues.2.The miR-27a-3p over-expression A549 cell line was successfully constructed by transfection with miR-27a-3p mimics.The results of RT-qPCR showed that the expression of mi R-27a-3p was significantly increased in the transfection group compared with the control group.At the same time,mi R-27a-3p-silenced A549 cell line was successfully constructed by transfection with mi R-27a-3p inhibitor.The results of RT-qPCR showed that the expression of mi R-27a-3p was significantly decreased in the transfection group compared with the control group.2.The results of MTT assay showed that the proliferation ability of A549 cells were enhanced significantly after overexpression of miR-27a-3p.On the contrary,after silencing the expression of miR-27a-3p,the proliferation ability of A549 cells was significantly reduced.3.The results of cell scratch assay showed that the migration ability of A549 cells were enhanced significantly after overexpression of miR-27a-3p.On the contrary,after silencing the expression of miR-27a-3p,the migration ability of A549 cells was significantly reduced.ConclusionCompared with normal tissues,lung adenocarcinoma tissues have high expression of miR-27a-3p.Overexpression of miR-27a-3p enhanced the proliferation and migration ability of lung cancer cells.The proliferation and migration of tumor cells are positively related to their malignancy.It suggests that miR-27a-3p is an oncogene that promotes the development of lung adenocarcinoma.Therefore,miR-27a-3p may be an important regulatory factor in the development of lung adenocarcinoma.These findings could not only further our understanding of human lung adenocarcinoma pathogenesis,but also facilitate the development of novel therapeutic target for lung cancer patients.