Expression And Clinical Of FOXC2 In Epithelial Ovarian Cancer

Objective: To investigate the expression and characteristic of FOXC2 and vasculogenic mimicry in epithelial ovarian cancer,analyze the correlation between them.By inhibiting the expression of FOXC2 in epithelial ovarian cancer cells,the effects of FOXC2 on the invasion,migration and tubing capacity of ovarian cancer cells were examined,to explore the effect and clinical significance of FOXC2 in epithelial ovarian cancer.Methods: The expression of FOXC2 and VM in normal ovarian tissue and epithelial ovarian cancer was detected by immunohistochemistry and CD34/PAS staining,and the correlation between them was studied.The expression of FOXC2 in SKOV3 ovarian cancer cells was also inhibited by si RNA technique,and the effects of cell scratch test.The expression of m RN A of FOXC2 in ovarian cancer cells was detected by RT-PCR.Transwell chamber invasion test and Matrigel stromal adhesive tube test were observed on the invasion,migration and capacity of SKOV3 ovarian cancer cel s.Results: 1.The positive expression rate of FOXC2 was 13.33% in normal ovarian tissues and 94.44% in epithelial ovarian cancer tissues.The expression of FOXC2 in epithelial ovarian cancer was significantly higher than in normal ovarian tissue,and the difference was statistically significant(P<0.05).2 VM was not found in normal ovarian tissues,but the positive expression rate was 31.48%.The expression of VM in epithelial ovarian cancer was significantly higher than in normal ovarian tissue,and the difference was statistically significant(P<0.05).3.The expression rate of FOXC2 and VM in epithelial ovarian cancer is closely related to the epithelial ovarian cancer stage,the differentiation status and lymph node transfer(P<0.05).4.The expression rate of FOXC2 in epithelial ovarian cancer was positively correlated with the expression of VM(P=0.033 r=0.299).5.While the FOXC2 gene in ovarian cancer cells was targeted by si RNA,The expression of FOXC2 m RNA in experimental group was significantly lower than that in blank control group and negative control group,the difference was statistically significant(P<0.05).6.Cell scratch test: the cell scratch width of the experimental group was larger thanthat of the blank control group and the negative control group(P<0.05),and the scratch width of the blank control group and the negative control group was about the same.7.Transwell chamber invasion test: the number of perforating cells in the experimental group was smaller than that in the negative control group and the blank control group(P<0.05),and the number of perforated cells in the blank control group and the negative control group was basically equal.8.Matrigel matrix glue tube forming experiment: the number of tubes formed in the experiment was less than that in the blank control group and the negative control group(P<0.05),while the number of tubes observed in the negative control group and the blank control group was basically the same.Conclusion: 1.The overexpression of FOXC2 and VM in human epithelial ovarian cancer suggests that FOXC2 may be effected the genesis and development of epithelial ovarian cancer.2.The expression of VM was positively correlated with the expression of FOXC2 in epithelial ovarian carcinoma,suggest that FOXC2 may be a factor to promote the formation of VM.3.After si RNA technology was used to inhibit the expression of FOXC2 in SKOV3 cells,the ability of ovarian cancer cells to migrate,invade and tube formation was reduced.The results suggested that VM formation was inhibited in ovarian cancer after FOXC2 expression,which decreased the migration and invasion of ovarian cancer.