IL-13 Regulates Human Nasal Epithelial Cell Differentiation Via H3K4me3 Modification

Part ? The effect and expression of H3K4me3 protein in CRSw NPsObjective:This study was conducted to examine the protein levels of H3K4me3 in nasal polyp tissues and normal inferior turbinate tissues,in order to further explore the role which H3K4me3 plays in the development of nasal polyps.Methods:From March to December in 2016,we collected 11 patients diagnosed with nasal polyps and evaluated the severity by Lund-Kennedy.We divided these patients into two subjects,and examined the m RNA levels of cilia-related transcription factors FOXJ1 and DNAI2 and goblet cell-derived genes CLCA1 and MUC5 a by RTPCR.Furthermore,we detected protein levels of H4K4me3 and analyze datas by Prime 6.0 software.Results:The patients with nasal polyps had expressed a higher protein level of H3K4me3,compared with normal inferior turbinate tissues according to western blotting.Also,RT-PCR showed relative FOXJ1 and DNAI2 m RNA expressed lower,while MUC5 a and CLCA1 m RNA expressed higher in nasal polyps tissues,compared with normal inferior turbinate tissues.Conclusion:It's likely that H3K4me3 may play an important role in the development and maintain of nasal polyps,which cloud be a novel targeted point to treat with CRSw NP.Part ? The expression of H3K4me3 in human nasal epithelial cells with IL-13 stimulationObjective:The aim of this study was to investigate nasal epithelial cells differentiation and explore the results of H3K4me3 and MLL1 after IL-13 stimulation,which could provide a novel therapy for CRSw NPs.Methods:We estimated the expression levels of H3K4me3 and MLL1 proteins and associated m RNA using reverse transcription-polymerase chain reaction(RT-PCR)assays and Western blot in human nasal epithelial cells before and after IL-induced respectively.Then after knockdown MLL1,we evaluated expression levels again.Moreover,we compared these results by statistical methods.Results:We observed that expression of H3K4me3 and its methyltransferase MLL1 was significantly upregulated in IL-13-treated HNEp C.It showed that m RNA expressions of cilia-related transcription factors FOXJ1 and DNAI2 decreased,while goblet cellderived genes CLCA1 and MUC5 a increased upon IL-13 treatment.After knockdown MLL1,theses m RNA expressions related to cell differentiation showed reversely expressed in comparison with without knockdown MLL1.Conclusion:These findings show that H3K4me3 is a critical regulator in control of nasal epithelial cell differentiation,suggesting that MLL1 may be a potential therapeutic target for nasal inflammatory diseases.