The Reactivation Of P53 By SaRNA Affects The Biological Behavior In Vitro In Gastric Cancer Cell

PurposeThis study attempts to reactivate the p53 gene in gastric cancer cells by RNA activation effect technique,and study the biological behavior of saRNA transfected in gastric cancer cells MGC-803 and BGC-823 after gastric cancer cells,and further clarify RNAa pairs.The influence of malignant tumor cells on biological behavior both inside and outside is a new direction for gene therapy of gastric cancer.MethodFind the literature to find out the effective saRNA sequence of p53 gene,namely,dsP53-285,and dsControl(negative control sequence)and synthesize,design the corresponding siRNA for P53 gene,namely si P53,in order to eliminate the Miss effect.According to the best transfection time concentration and time of the known literature,dsP53-285 was transfected into gastric cancer cells MGC-803 and BGC-823 as the experimental group,while the negative control sequence(dsControl)was transferred into the human stomach MGC-803 and BGC-823,and dsControl had no corresponding homologous sequence or complementation in the current known human gene sequences.The transfected target cells were used as negative control group.In addition,for the completeness of the experiment,we also designed the target cells with only the transfection reagents,as a blank group,mock group,and then detected the protein expression level of p53 in each group of gastric cancer cells by Western Blot.The expression of P53 based factors in MGC-803 and BGC-823 in gastric cancer cells was detected by RT-qPCR,and the activation efficacy of dsP53-285 was verified.Yes.Subsequently,cell proliferation assay was used to detect the effect of dsP53-285 on the growth of target cells.In this experiment,siP53 was used to exclude the Miss effect of dsP53-285;the changes in the migration ability of MGC-803 andBGC-823 cells in the stomach were observed and evaluated by scratch test.The role of siP53 was in the same front.Result1.dsP53-285 can effectively upregulate MGC-803 and BGC-823P53 gene expression in human gastric cancer cells,that is,increase the expression of P53 gene mRNA and protein.2.dsP53-285 can inhibit the proliferation of target cells by up regulating the P53 gene expression of MGC-803 and BGC-823 cells.3.dsP53-285 can effectively up regulate the expression of MGC-803 and BGC-823P53 genes and inhibit the migration of target cells in human gastric cancer cells.ConclusionWe found that dsP53-285 could reactivate P53 gene expression and increase the content of p53 protein and mRNA,thereby inhibiting the malignant behavior of gastric cancer cells.In other words,it is feasible to increase the expression of the target gene by saRNA to suppress the biological behavior of malignant tumor and to provide a new method and treatment for the prevention and treatment of gastric cancer and other malignant tumors by saRNA and RNAa.