Abnormal Expression Of Clusterin And Stathmin2 In Epileptic Patients And Rats

ObjectiveTo detect the expression of Clusterin and Stathmin2 in brain tissue of epileptic patients and rats.MethodsCortical samples of epileptic patients were collected as epilepsy group(n=20).Cortical samples of epileptic rats were collected as control group(n=20).100 SD rats underwent injection of pentetrazole(PTZ)intraperitoneally every second day for 15 injections.The rats that underwent 24 h after first injection of PTZ were collected as EP1 group(n=20).The rats that underwent 24 h after fifth injection of PTZ were collected as EP2 group(n=20).The rats that underwent 24 h after tenth injection of PTZ were collected as EP3 group(n=20).The rats that underwent 24 h after fifteenth injection of PTZ were collected as EP4group(n=20).20 rats undergoing no treatment were collected as EP0 group.The altered expression of Clusterin and Stathmin2 were analyzed by immunofluorescence,immunohistochemistry,western blot and co-immunoprecipitation.ResultsImmunofluorescence indicated that Clusterin and Stathmin2 mainly located in neuronal cytomembrane and cytoplasm.Both immunohistochemistry and western blot indicated that the expression of Clusterin was decreased and the expression of Stathmin2 was increased in the epileptic patients and epileptic rats(P<0.05).Co-immunoprecipitation indicated that the binding of Clusterin to Stathmin2 was decreased(P<0.05).ConclusionAbnormal expression of Clusterin and Stathmin2 and abnormal binding of Clusterin to Stathmin2 may be involved in the epileptogenesis.