The Expression And Significance Of IGF2 And Autophagy Related Proteins In Villus Of Embryonic Death

Objective: To detect the expressions of insulin-like growth factor 2(IGF2),Beclin1 and mammalian target of rapamycin(mTOR)in normal early villus and the villus of embryoic death patients and to explore the relationship among the expression of the three proteins in villus.Methods:Using immunohistochemical method to detect the expression of IGF2,Beclin1 and mTOR in 48 cases of embryonic death villus samples and 40 cases of normal early villus samples,and to analyze the relationship between their expressions and the development of embryonic death.Results The positive expression rates of IGF2,Beclin1 and mTOR were significantly lower in embryonic death villus than those in normal early villus(P=0.004,P=0.000,P=0.000).In normal early villus,there were significantly positive correlations between IGF2 and Beclin1 and mTOR.No correlation was found between Beclin1 and mTOR in normal early villus.In embryonic death villus,significantly positive correlation was also detected between IGF2 and Beclin1.While no correlation was detected among IGF2,mTOR and Beclin1 in embryonic death villus.Conclusions The IGF2 regulated autophagy might have some relationship with early villus development.In embryonic death villus,the lower expression of IGF2 might led to the loss of its regulation to autophagy through m TOR pathway,and which might lead to the autophagy disturbance and early embryonic death.