| Objective:To explore whether there is gender difference in effects of acupuncture treatment of Diarrhea-Predominant irritable bowel syndrome(IBS-D)and to explore the correlation between the differences and sex hormones.Methods:70(half male and half female)adult Sprague Dawley(SD)rats were excluded from the baseline assessment,then divided into blank control group,model group,and acupuncture intervention group by different sex.Rats in the model and acupuncture group were treated with senna extract gavage combined with Chronic unpredictable mild stress(CUMS)modeling mode for 14 days.After the completion of the model,the model was judged and compared with the IBS-D symptoms in the female model rats and the male model rats,to verify the gender difference in the IBS-D pathogenesis.Then the acupuncture group started the intervention with that acupoints,ST25,ST36,SP6,and LR3,by bilateral alternate were performed,for 14 days.Once a day,each needle was retained for 20 minutes.After the intervention,compared to the improvement about the symptoms of IBS-D related indicators,include the general situation,the intestinal conditions,anxiety and depression indicators of rats.At the same time,the corresponding sex hormone concentration in blood of the blank,model,and acupuncture group rats were detected by enzyme-linked immunosorbent assay(ELISA),and detected the corresponding sex hormone receptor positive rate in the intestine by immunohistochemistry.And performed correlation analysis with IBS-D symptoms.Results:l.At the baseline period,there was no gender difference in the food intake in healthy rats(P>0.05),but the weight of male rats increased significantly faster than that of female rats(P<0.05).There was also no gender difference in visceral sensitivity,diarrhea,anxiety and depression in healthy rats(P>0.05).2.After the model was established,through comparing with the blank group,no matter male and female rats,the diarrhea index was elevated(P<0.05),and the visceral sensitivity was also significantly higher(P<0.05).The open arm time percent(OT%)and sucrose preference(SP)were significantly decreased(P<0.05),indicating anxiety and depression.Through the comparison between male and female model rats,the female rats' food intake and body weight were significantly lower than those of male rats(P<0.05).The female rats had significantly lower Abdominal withdrawal reflex(AWR)values than those of male rats(P<0.05).The SP of the female model rats was significantly higher than that of the male model rats(P<0.05).3.After the intervention of acupuncture,the diarrhea index of the acupuncture group was significantly lower than that of the model group,and the AWR values were significantly higher than that of the model group.OT%and SP were higher than the model group(P<0.05).The male and female rats were found to have no gender difference in the amount of food intake,but the weight of the male rats was still higher than that of the female rats(P<0.05).Acupuncture intervention improved the visceral sensitivity of male rats better than female rats(P<0.05),but diarrhea index and anxiety and depression improvement did not have gender differences(P>0.05).4.The levels of estradiol(E2)in the blood of female model rats in the model group were significantly lower than those in the blank group and the acupunctured female rats(P<0.05).The levels of testosterone(T)in the male rats of the model group were significantly lower than those in the control group and Acupuncture group rats(P<0.05).The estrogen receptor(ER)positive cells in the model group were significantly higher than those in the blank group and the acupunctured female group(P<0.05).The androgen receptor(AR)receptors in the male rats of the model group was significantly higher than that of the blank group and the acupunctured group(P<0.05),and there was no significant difference in the number of intestinal AR positive cells between the acupuncture group and the blank group(P>0.05).5.There was a significant positive correlation between E2 concentration and visceral sensitivity in female blood(P<0.05).There was also a significant positive correlation between serum T concentration and visceral sensitivity in male rats(P<0.05);There was a significant negative correlation between the counts of ER-positive cells in female rat's intestine and visceral sensitivity(P<0.05),and there was a significant negative correlation between AR counts in male rats and visceral sensitivity in male rats(P<0.05).Conclusions:1.It can be confirmed that there are gender differences in the severity of IBS-D symptoms.2.The intervention effect of acupuncture on IBS-D model rats is precise,and there is a gender difference in the improvement of visceral sensitivity,the male better than female.3.Gender differences in IBS-D disorders are closely related to sex hormones and their receptors,and sex differences in acupuncture effects are also related to sex hormones and their receptors. |
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