Quantitative Study On Gut Microbiota Of Mother And Infant Based On Droplet Digital PCR

The gut microbiota in mother and infant has always been a research hotspot.With the deepening of research,researchers have discovered that newborns carry a large number of bacteria from the moment they were born.Delivery mode is an important factor influencing the establishment of the gut microbiota in infancy.However,there are few studies focus on the effect of delivery mode on the number of gut microbiota in infants.And the study on the effect of maternal childbearing age on the number of gut microbiota in infants is rarely reported.In this study,the maternal and infant fecal samples were studies from 14 mothers and infants,the total number of bacteria and core microbiota such as Enterobacteriaceae,Lactobacillus,Bifidobacterium,Bacteroides,Clostridium,Prevotella,Streptococcus,Lactococcus,Enterococcus and Escherichia coli in feces were quantified using the droplet digital PCR.The main findings are as follows:(1)Droplet digital PCR technology has the advantage of high sensitivity,which can be used for quantitative detection of intestinal microbiota in newborn infants.(2)There was a significant difference in the total number of bacteria present in the gut microbiota of infants with different delivery modes(p<0.05).The amounts of Enterobacteriaceae and lactobacillus in the infants who were given birth naturally were significantly higher than those underwent caesarean section(p<0.05),indicating that delivery mode affects the transmission of microbes from mother to infant(3)There was no significant difference in the number of these core gut microbiota between mothers.However,the amounts of Streptococcus in the meconium of infant who given birth from the elder mothers was significantly higher than those from the young mothers(p<0.05),indicating that the maternal childbearing age may have an effect on the transmission of streptococcus...