Expression Of CXCL13 And Its Receptor CXCR5 In Gastric Cancer Tissues And Its Correlation With Lymph Node Metastasis

Objective:Investigate the CXC chemokine ligand 13(also known as B-lymphocyte chemoattractant,BLC/CXCL13)and the CXC chemokine receptor 5(also known as Burkholder’s lymphoma receptor 1,BLR-1/CXCR5)expression in primary gastric cancer and lymph node metastasis and its correlation with lymph node metastasis.Methods:A total of 100 cases of paraffin-embedded specimens of pT3 and pT4 advanced radical gastrectomy were collected from Qingdao Municipal Hospital from 2015 to 2016.100 cases were selected,and all of them had gastric cancer tissues and paracancerous tissues(non-neoplastic 1 cm range beside the cancer tissue).In 100 cases of gastric cancer,57 cases had lymph node metastasis and 43 cases did not have lymph node metastasis.All the diagnosis results were confirmed by pathological diagnosis.Among 100 gastric cancer specimens,there were 59 male cases and 41 female cases,aged from 27 to 83 years old,with an average of 64.39 and 10.53.All selected cases were not treated with radiotherapy,chemotherapy or endocrine therapy before operation.Immunohistochemistry was used to detect the expression of CXCL13 and its receptor CXCR5 in gastric cancer tissues,paracancerous tissues and lymph node metastases.Statistical analysis was using SPSS22.0 software using Chi-square test statistical method and other methods.Result:1.The positive expression rate of chemokine CXCL13 and CXCR5 in gastric cancer was Significantly higher than paracancerous tissues(chi-square test,χ~2=16.092,38.095,P<0.05)2.The rate of lymph node metastasis in gastric cancer cases expressing chemokine CXCL13 was significantly higher than that of gastric cancer cases without CXCL13expression(Chi-square test,χ2=42.814,P<0.05).3.The expression of chemokine CXCL13 and its receptor CXCR5 in gastric cancer was not significantly associated with gender,age,staging,Laure type,depth of invasion,etc.(P>0.05).Conclusion:The chemokine CXCL13 and its receptor CXCR5 are abnormally expressed in gastric cancer tissues,The expression of CXCL13 in gastric cancer is related to the occurrence of lymph node metastasis,and may play a role in lymph node metastasis of gastric cancer.Significance:This article examines the expression of CXCL13 and CXCR5 in gastric cancer tissues,paracancerous tissues,and metastatic foci.From a new perspective,we can understand the role of chemokines in metastasis of gastric cancer.The results showed that the positive rate of chemokine CXCL13 and its receptor CXCR5 in gastric cancer tissues was higher than that in paracancerous tissues,and that chemokine receptor CXCR5 was expressed in gastric cancer tissues but not in adjacent tissues.Chemokine CXCL13 was found in gastric cancer.The positive correlation between the expression and the occurrence of lymph node metastasis suggests that both may become biomarkers for the pathological diagnosis of gastric cancer,and the chemokine CXCL13 may further become an important indicator to determine the lymph node metastasis and prognosis of gastric cancer.