Urinary Metabolomics For The Osteonecrosis Of The Femoral Head Patients Based On HPLC-QTOF/MS

Objective:Osteonecrosis of the femoral head(ONFH),one of the widespread orthopaedic diseases with a decrease in bloodstream to the femoral head,is frequently accompanied by cellular death,trabecula fracture,and collapse of the articular surface.The exactly pathological mechanism of ONFH is unknown.The early and rapid diagnosis biomarkers for this disease remain to explore and further identify.Methods:high performance liquid chromatography-quadrupole-time of flight tandem mass spectrometry(HPLC-Q-TOF/MS)in combination with multivariate statistical analysis was developed and performed to identify the metabolic profiling of urine from 26 ONFH patients and 26 healthy people.Results:28 distinctly differential metabolites were detected between the ONFH patients and healthy people.Dipropyl disulfide,trimethylselenonium and carbamic acid were expected to be the potential biomarkers of ONFH.In addition,the up/down-regulation of purine metabolism,tyrosine metabolism,fructose and mannose metabolism were clearly be associated with the ONFH pathogenic progress.The results of bioinformatics analysis suggested a disturbance in energy metabolism,skeletal development,bone metabolism,mineral metabolism,lipid metabolism,lipid oxidation,vascular permeability and oxidative stress of the ONFH patients.Conclusions:metabolomics could serve as a promising approach for identifying the diagnostic biomarkers and elucidating the pathological mechanism of ONFH.