Expression And Clinical Significance Of Girdin And Beclin1 In Epithelial Ovarian Cancer

Objective:Because of the unknown pathogenesis of ovarian cancer and the lack of effective detection methods in the early stage,the symptoms are not specific,and the mortality of ovarian cancer is the first in gynecological cancer.Although there have been lots of basic and clinical research,but patients with five year survival rate is only 30%.Therefore,a deeper understanding of the occurrence,development and invasion and metastasis mechanism of ovarian cancer,and exploring the mechanism of drug resistance and seeking new therapeutic regimens are particularly important.Girdin protein,a new actin in mammals,is also known as the protein kinase B(AKT)phosphorylated enhancer(APE)because it can promote the phosphorylation of AKT.AKT plays an important role in the growth,invasion and metastasis of tumor cells.Some studies have shown that Girdin protein is associated with the occurrence of tumor.At present,the relating tumors have been confirmed,such as cervical cancer,lung cancer,breast cancer,but the correlation with epithelial ovarian cancer has not yet been reported.It is reported that Girdin protein regulates the autophagy process under the action of growth factors.Autophagy keeps the cell in a normal physiological state,its abnormal function leads to the occurrence of tumor.Of the many autophagy relating genes,Beclin1 is the most important.As a tumor suppressor gene,Beclin1exists in epithelial ovarian cancer,osteosarcoma,lung adenocarcinoma and so on.Review of the literature,the relationship of them in epithelial ovarian cancer has not been reported.In view of this,through the research of Girdin and Beclin1 in normal ovarian tissues and ovarian cancer tissues,analyzing the relationship between them,to seek the mechanism of pathogenesis,development of ovarian cancer.Method:1 subjects and methods:Through the immunohistochemical method todetecte the expression of Girdin and Beclin1 in 82 cases of epithelial ovarian cancer paraffin tissue and 20 cases of normal ovarian paraffin tissue.2 data analysis:using SPSS21.0 statistical software,the expression of Girdin and Beclin1 in normal ovarian tissues and ovarian cancer tissues by using X~2 test,comparison between groups of epithelial ovarian cancer by X~2test,analysing the correlation between Girdin and Beclin1 by using Spearman rank correlation,P<0.05,the above are statistically significant.Result:1.The positive expression of Girdin in normal ovarian tissues was 20%(4/20),the positive expression in epithelial ovarian cancer tissue was 68.29%(56/82);the positive expression of Beclin1 in normal ovarian tissues was 80%(16/20),the positive expression in epithelial ovarian carcinoma was 36.58%(30/82),above were significantly.2.The expression of Girdin and Beclin1 in epithelial ovarian cancer was not correlated with the patients'age and histologic type,(P>0.05);It was related to the pathological stage,differentiation type and lymph node metastasis:the higher the pathological stage?the lower the differentiation degree and in lymph node metastasisthe tissue,the higher the positive rate of Girdin,and the lower the positive rate of beclin1(P<0.05).3.The expression of Girdin and Beclin1 in epithelial ovarian cancer tissue was a negative correlation(r=-0.407,P<0.05)Conclusion:1.Girdin is highly expressed in epithelial ovarian cancer and may be regulated as a proto oncogene in the occurrence and development of ovarian cancer;2.Beclin1 showed low expression in epithelial ovarian cancer;3.There is a negative correlation between Girdin and Beclin1 in ovarian cancer tissue.They may participate in the development of epithelial ovarian cancer.Combining detection is helpful for the early diagnosis and assessmen.