Development And Change Of Autophagy Of Human Ovarian Cancer Cells SKOV3during Chemotherapy

Objective:5-fluorouracil (5FU) is one of the most commonly usedantitumor drugs in clinical at present，to Inhibit the growth of cancer cellsby interfering metabolism of DNA. However, the mechanism of5-FU ismore complicated. clear mechanism has not yet been studied. Now, there areonly a little studies about autophagy in ovarian cancer cells SKOV3inclucedby the chemotherapy drug5-fluorouracil (5-FU). Our experimentation toexplore the effects and molecular mechanisms of inducing autophagy inovarian cancer cells SKOV3incluced by the chemotherapy drug5-fluorouracil (5-FU) and the inhibition effects of bafilomycin A1to theautophagy of ovarian cancer cells SKOV3handled by5-fluorouracil(5-FU)and the possible mechanism of enhancing chemotherapy sensitivity.Methods: Formation of autophagy after the treatment of5-FU (50μmol/L,48h) to ovarian cancer cells SKOV3and Ovarian cancer cellsSKOV3were administered for1h with100nmol/L bafilomycin A1,Thentreated for48h with50aμmol/L5-fluorouracil were observed with acridineorange staining and indirect immnnofluotesent assay. Meanwhile, the expression levels of LC3and Beclin1in SKOV3cells were also detected byWestern blotting.Results: Acridine orange staining showed that5-FU treatmentmarkedly increased the acid areas in SKOV3cells. And theimmnnofluotesent assay showed5-FU treatment increased the formation ofautophagy. Furthermore, the expressions levels of Beclin1and LC3proteinsin SKOV3cells were significantly increased after the treatment of5-FU;Acidine orange staining showed that the acid areas were markedly decreasedin SKOVE cells treated of bafilomycin A1. And the immnnofluotesent assayshowed the treatment of bafilomycin A1weakened the formation ofautophagy. Furthermore, the expressions levels of LC3and Beclin1Proteinsin SKOV3cells were significantly reduced after the treatment ofbafilomycin A1.Conclusion:5-FU might induce the autophagy of SKOV3cellsthrough increasing the acidic areas and up-regulating the expression ofBeclin1and LC3proteins in SKOV3cells;Bafilomycin A1might inhibit theautophagy of SKOV3cells by reducing the number of acid areas in SKOV3cells, and inhibiting the expression of LC3and Beclin1proteins in SKOV3cells.