The Expression Of LIGHT And Its Receptors In Scleroderma,keloid And Lichen Sclerosus Et Atrophicus

Objective:Scleroderma is a connective tissue disease characterized by skin and visceral fibrosis.There are many factors involved in the pathogenesis of the disease,among which autoimmune is playing an important role in the pathogenesis of scleroderma;Lichen sclerosus et atrophicus is a chronic,inflammatory disease,which can cause fibrosis and sclerosis of the skin or viscera,and its pathogenesis is related to autoimmune factors;Keloid is a disease of the abnormal aggregation of collagen fibers and the formation of scar.Its etiology is related to genetic factors,wound tension and dysregulation of cell apoptosis.The abnormal deposition of fibroblasts and collagen plays an important role in the formation of keloids.LIGHT?Lymphotoxin-like,exhibits inducible expression,and competes with HSV glycoprotein D for HVEM,a receptor expressed by T lymphocytes?is an important member of TNF superfamily,also known as TNFSF14,belonging to a lymphoid analogues.LIGHT and its receptors play important biological functions in many diseases,especially in autoimmune diseases^[1].LIGHT can participate in tissue fibrosis and remodeling,interact with its receptors in inflammatory or structural cells to participate in the expression of some fibrotic factors and promote the deposition of extracellular matrix proteins such as collagen^[2].However,the role and significance of LIGHT in skin tissue of scleroderma,keloid,and lichen sclerosus et atrophicus are not reported.To investigate the expression and significance of LIGHT and its receptor LT?R and HVEM in scleroderma,keloid and sclerosing lichen scleroses,The expression level of LIGHT and its receptor protein in three kinds of disease tissues was compared laterally,and the pathophysiological significance of LIGHT and its receptor was preliminarily discussed.Methods:The First Affiliated Hospital of China Medical University?Department of Dermatology?were diagnosed scleroderma,keloid,lichen sclerosis tissue paraffin specimens of 20 cases,the average age was 40±21,41±19,46±17 years old;The First Affiliated Hospital of China Medical University?plastic surgery?,15 cases of normal skin tissues,the average age of 43±11 years old;Immunohistochemical method for LIGHT and LT?R and HVEM in scleroderma,keloid,the expression of lichen sclerosis atrophy group and normal skin tissues.Results:LIGHT was negative in normal skin tissues,and was positive in scleroderma,lichen sclerosus,keloid,dermal fibroblasts,vascular endothelial cells and inflammatory cells.HVEM and LT?R showed weakly positive expression in dermis of normal human skin,and were strongly positive in scleroderma,lichen sclerosus,keloid,dermal fibroblasts,vascular endothelial cells and inflammatory cells.The expression of LIGHT protein in lichen sclerosis tissues was higher than that of scleroderma organization,expression in scleroderma tissues than in keloid tissue;the expression of HVEM protein in lichen sclerosis tissues was higher than that of keloid tissue expression in keloid tissue high in scleroderma tissues;the expression of LT?R protein in keloid tissues were significantly higher than that in scleroderma tissue,expression in scleroderma tissues was higher than that of lichen sclerosus organization.Conclusion:Immunohistochemical results suggest that LIGHT and its receptors LT?R and HVEM may be involved in the pathogenesis of scleroderma,keloid and sclerosing atrophic lichen,but the specific form and path of their participation need further confirmation.