The Study On Relationship Between Vulvar Lichen Sclerosus And Hla-dqb1 Genes

Objective:Human leucocyte antigen complex(HLA) ,the most complicated and polymorphic inheriting system in human gene groups,has beendiscovered to be associated with more than 500 kinds diseases.This research aims at finding susceptibility or antagonistic gene locations of HLA- DQB1 locus by detection of polymorphism of HLA- DQB1 genes,so as to prove the correlation between vulvar lichen sclerosus(VLS) and some of HLA- DQB1 gene,thus laying some scientific basis for disclosing the mechanism.Methods: 27 female patients whose pathological diagnosis was vulvar lichen sclerosus were chose,from 25 to 64 ages, the average age was 44.3.They were not related to each other.; The control group consist of 30 physical healthy females, from 22 to 65 ages,the average age was 44.6. They were not related to each other. too..The genotypes of HLA-DQB1 in Peripheral blood of 30 healthy controls and 27 patients with vulva lichen sclerosus were determined by polymerase chainreaction sequence specific primer. The relationship between vulva lichen sclerosus and frequencies of HLA-DQB1 alleles was analyzed using genetic statistical methodsResult : After testing the selected samples met the Hardy-Weinberg equilibrium law. During the test, the negative control was set up and DNA Maker was added,so that the test results are accurate and reliable.The frequency of HLA-DQB1*02,03 allele was significantly higher in patient group(0.22) than in control group(0)( p<0.05).However, the frequency of HLA-DQB1 allele was significantly lower in patient group(0.05) than in control group (0.23)(p<0.05).Conclusion:HLA-DQB1*02,03 and HLA-DQB1*06 allele may be the susceptibility and resistant genes in the patients with vulva lichen sclerosus respectively.