Study On Immunosuppressive Activity And Metabolism Of Calceolarioside A

An epidemiological investigation of allergic diseases by the World Allergy Organization showed that 22%of people were suffering from allergic diseases.Allergic reaction refers to the reaction of tissue damage or parafunction that occurs when the body that has been immunized is stimulated by the same antigen again.The allergic reaction is largely caused by the presence of specific IgE,which is specific to a certain allergen,in the blood.In this paper,the immunosuppressive activity of calceolarioside A was studied by simulating the body's immune environment through culturing the cells in-vitro.In addition,the pharmacokinetics of calceolarioside A in mice and the possibility of administering orally by intragastric administration were explored.The experiment results showed that calceolarioside A had a significant inhibitory effect on the process of U226 cells secreting IgE,with IC50 value of9.25?g/mL;and it inhibited the process of RAW264.7 cells excreting TNF-?,with IC50 value of 120?g/mL,which showed that it had a potential anti-inflammatory effect;it also had an obvious inhibitory effect on the process of mouse splenocytes secreting IL-2,with IC50 value of 14.95?g/mL.After a single oral administration?50 mg/kg?,the half-life of calceolarioside A in mice was T1/2=4.46±0.79 h,Tmax=1 h,and the maximum plasma concentration was C_max=661.19±104.07 ng/mL.The above experimental results indicated that calceolarioside A had potential immunosuppressive effects,and it could be administered orally.The results of this experiment would provide experimental basis for developing calceolarioside A into an oral immunosuppressant as well as treating of immune diseases.