The Changes And Significance Of Serum NSE And BDNF In Premature Infants With NRDS

Objective:To study the changes of serum neuron-specific enolase and brain-derived neurotrophic factor in premature infants with neonatal respiratory distress syndrome in different period,and provide the norm for reference to the early diagnosis of brain injury in premature infants with neonatal respiratory distress syndrome.Methods:From February 19,2016 to December 31,2017,80 newborns in the Department of Neonatrics,second affiliated Hospital of Kunming Medical University,were selected as the study subjects.The inclusion criteria in the case group:premature infants with gestational age<37 weeks and more than 28 weeks within 12 hours were clearly diagnosed neonatal respiratory distress syndrome.All the participants received 1.5 ml femoral venous blood within 12 hours?48 hours and 72 hours after admission.In the control group,there were 30 cases of premature infants who had no dyspnea,asphyxia and premature rupture of the membranes during the same period.They were all received 1.5 ml of femoral venous blood within 12 hours of admission.The serum NSE was detected by Roche electrochemiluminescence analyzer and its associated reagents,and the serum BDNF was detected by enzyme linked immunosorbent assay.We observed the state of illness and prognosis,recorded the gestational age,body weight,sex,delivery mode,body temperature,respiration,heart rate,nervous system manifestation,blood glucose,blood gas analysis,craniocerebral B-ultrasound SNAPPE-? results,and then,we done data consolidation and statistical analysis.Results:The serum NSE and BDNF in NRDS group were significantly higher than those in control group within 12 hours after admission,and the differences were statistically significant(P<0.05);In 12 hours,48 hours and 72 hours after admission,the serum levels of NSE and BDNF in NRDS group showed a decreasing trend,and the differences were statistically significant(P<0.05);The higher the SNAPPE-II was,the higher the serum of NSE and BDNF was within 12 hours after admission,the difference was statistically significant(P<0.05),and there was a low positive correlation between the two groups;The longer the ventilator we used,the higher the serum of NSE and BDNF would be,and the difference was statistically significant(P<0.05),There was a low positive correlation between two groups;The longer the abnormal results of skull cavity ultrasound sustained,the higher the serum of NSE and BDNF would be,the difference was statistically significant(P<0.05).Conclusion:1.The level of serum NSE in premature infants increased at the beginning of NRDS,then decreased,suggesting that premature infants had transient brain damage caused by hypoxia in the early stage of NRDS,and then most of the children with NRDS were quickly corrected after effective treatment,and no permanent brain damage was caused.At the same time,the BDNF was increased during brain injury.2.Serum NSE and BDNF combined with SNAPPE-? is helpful for diagnosing early brain injury of the premature infants with NRDS.3.Some severe NRDS premature infants can not be corrected from hypoxia,or it will take long time in ventilator assisted ventilation,which can easily cause premature brain damage.