| Objectives:The Jagged-2/Notch-1/Hes-1 signaling plays a vital role in the maintenance of intestinal crypt epithelial cell regeneration,but its role in intestinal epithelial cell after IAH/ACS injury is poorly understood.This study investigated the role of Jagged-2/Notch-1/Hes-1 signaling on the regeneration of intestinal crypt epithelial cells in abdominal compartment syndrome(ACS)rat model.Methods:1.The establishment of ACS model:a rat model of abdominal compartment syndrome(ACS)was established by using a simple intra abdominal pressure gauge based on a Jiangsu Yuyue platform sphygmomanometer;2.Animal grouping and surgical treatment:30 male SD rats were randomly divided into sham group and ACS 0.5h,2h,4h,6h groups(6 rats in each group).the rats in the sham operation group,of which only the operation process was performed,and no intra-abdominal pressure treatment was performed;ACS groups was induced by exposing to IAP under nitrogen pneumoperitoneum of 20-22 mm Hg for 0.5,2,4 or 6 hours,of which the pressure measuring device was connected with the abdominal cavity in the left lower abdomen and injected with a certain amount of nitrogen,and the IAP was dynamically monitored and maintained until the scheduled time;3.Detection of content and methods:Each group was killed at the corresponding time;then structural injury to the ileal mucosa at different hours of ACS was confirmed by light electron microscopy;moreover,the expression changes of Jagged-2/Notch-1/Hes-1 signaling components Jagged-2,Notch-1,Hes-land Math-1 mRNA levels and Jagged-2,NICD1,Hes-1 protein levels of the ileal mucosa were tested by Q-PCR and Western blot analyses;finally,immunofluorescence was used to detect the changes of Ki-67 and Math-1 in the ileal mucosa.Results:1.Microscopic observation showed that there was no obvious damage to the ileal mucosa in the sham operation group,which showed that the normal columnar epithelium was optical;however,in the ACS groups,extensive ileal villus damage was exposed to prolonged intra-abdominal pressure;2.IAH/ACS injury was shown the mRNA expressions for Jagged-2,Notch-15 Hes-land Math-1 and protein expressions for Jagged-2,NICD1,Hes-1,which performances were increased first and then reduced;3.Immunofluorescence staining confirmed the expressions of proteins of Ki-67 and Math-1 in the intestinal crypt epithelial cells according to morphology.Conclusions:Intestinal mucosal injury occurred early in IAH/ACS,and the proliferation of intestinal epithelial cells appeared in the early stage of injury,and it was closely related to the activation of Jagged-2/Notch-1/Hes-1 signaling pathway. |
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