Study Of IVIM And DKI MRI In Evaluating Renal Ischemia Reperfusion Injury In Rats

Part ? Preliminary Study of IVIM MRI in Evaluating Renal Ischemia Reperfusion Injury in RatsObjectivesTo investigate DWI-IVIM technique tracking renal IRI process in rats.To demonstrate the changes of IVIM parameters in IRI model initially and compare the efficacy of various parameters in the diagnosis of renal injury.Materials and MethodsRenal IRI models were established by clamping bilateral renal arteries for 45 min at the same time in SD rats.Control groups and eight rats randomly selected from model groups at time point 1h,1d,3d,7d,14d,and 28d were performed MRI scan.The venous blood was taken immediately and kidney tissues were sacrificed for pathological examination.The D value,D*value,f value and ADC value of the outer cortex,OSOM and ISOM were measured.IVIM parameters of each layer and laboratory results were statistically analyzed with pathological results.ResultsExcept for group 1d,creatinine and urea of the other model groups were not statistically different from the control group.In the renal cortex and the OSOM,D value and ADC value of every model group were statistical different from control group(P<0.05);the f value of the renal cortex in group lh-7d was lower than that in the control group(P<0.05);there was no statistical difference between the D*and the control group.In ISOM,compared with the control group,D value of each model group decreased(P<0.05);except for the 1h,3d,and 7d groups,ADC values of the other model groups were not statistically significant with the control group;only the f values of 1h and 3d groups were significantly different when compared to the control group,and the D*value was lower than that of the control group at 1 hour(P<0.05).There was a high negative correlation between D value and pathological score,low correlation between pathological score and f value in OSOM and ISOM.Pathological score was moderate correlated with D value and ADC value in CO and ISOM,as well as ADC value in OSOM.ConclusionMagnetic resonance DWI-IVIM imaging has a high value in monitoring the progress of renal ischemia-reperfusion injury in rats and judging the degree of renal injury.Part ? Preliminary Study of IVIM MRI in Evaluating Renal Ischemia Reperfusion Injury in Rats and comparative study with IVIMObjectivesTo investigate DKI technique tracking renal IRI process in rats.To demonstrate the changes of DKI parameters in IRI model initially and compare the efficacy of various parameters in the diagnosis of renal injury.Materials and MethodsRenal IRI models were established by clamping bilateral renal arteries for 45 min at the same time in SD rats.Control groups and eight rats randomly selected from model groups at time point 1h,Id,3d,7d,14d,and 28d were performed MRI scan.The venous blood was taken immediately and kidney tissues were sacrificed for pathological examination.The DDKI value,fa value and MK value of the outer cortex,OSOM and ISOM were measured.DKI parameters of each layer and laboratory results were statistically analyzed with pathological results.ResultsExcept for group 1d,creatinine and urea of the other model groups were not statistically different from the control group.In renal cortex,the DDKI values of group lh-7d were statistically different from that of the control group,but there was no significant difference in fa values between the control group and every model group.In OSOM,except for group 14d,DDKI values of the other model groups were lower than that of the control group(P<0.05),and fa values of model groups were significantly lower than that of the control group before day 14(P<0.05).In ISOM,DDKI values of group lh-28d were statistically different from the control group(P<0.05);the fa values of the 1h,3d,and 7d groups were statistically different from that of the control group.MK values were not statistically significant compared to the control group in cortex,OSOM and ISOM.Fa values and the pathological scores in OSOM and ISOM showed a moderate negative correlation,while DDKI values showed a low negative correlation with the pathological scores.When distinguishing the differences between the model groups and between different pathological scoring groups,the efficiency of ADC values was superior to DDKI values.When distinguishing between control group and higher than 2,3 score groups,the parameter with the highest efficiency in the cortex,OSOM and ISOM were D value,D value,and fa value.When distinguishing between control group and higher than 4 score groups,the parameter with the highest efficiency in the cortex,OSOM and ISOM were ADC value,D value,and fa value.ConclusionMagnetic resonance DKI imaging has a high value in monitoring the progress of renal ischemia reperfusion injury in rats and judging the degree of renal pathological injury.IVIM is more accurate than DKI in the diagnosis of renal IRI in rats.The combination of two techniques is more effective.