Correlation Between CYP3A5 And VEGFR3 Gene Polymorphism And Clinical Characteristics Of Renal Carcinoma Patients

Objective:In this paper,the correlation between CYP3A5 and VEGFR3 gene polymorphism in patients with renal carcinoma was analyzed and its clinical significance was discussed.Methods:Collect the clinical information of 34 patients who underwent radical nephrectomy or nephron sparing surgery and whose postoperative pathology was diagnosed as renal cell carcinoma from the second Affiliated Hospital of Kunming Medical University during December 2015 and September 2017.After the renal cell carcinoma was confirmed by pathological findings,we extracted the peripheral blood fluid of the patients for gene detection and then the results was collected.According to selected patients with kidney cancer gender,age,tumor site,tumor staging and pathologic type group,observe the SNPs rs307826(VEGFR3 gene)and rs776746(CYP3A5 gene)gene polymorphisms in the difference of each group for statistical analysis and comparison.Results:1.The results of the VEGFR3 gene rs307826 polymorphisms showed that all the 34 patients who were selected were wild-type homozygote A/A,and did not see the variant heterozygote A/G and the variant homozygous G/G.There was no significant correlation between the gender,age,tumor location,tumor staging and pathological type of rs307826 genotype and renal cancer patients.2.The results of CYP3A5 gene rs776746 polymorphisms showed that there were 19 cases of wild homozygous G/G,14 cases of variant heterozygote G/A,1 case of variant homozygote A/A in the 34 patients who were selected.Statistical analysis showed no statistically significant differences in the gender,age,tumor site,tumor staging and pathological type of rs776746 site genotype and renal cancer patients(P>0.05).3.There is a higher probability of wild-type homozygote A/A in VEGFR3 gene rs3 07826 polymorphisms of renal carcinoma patients in this region than in Spain(P=0.014).There was a statistical difference between the two groups.4.There is a higher probability of variant heterozygote G/A in CYP3A5 gene rs776746 polymorphisms of renal carcinoma patients in this region than in Spain(P<0.01).There was a statistical difference between the two groups.Conclusion:1.The distribution of VEGFR3 gene rs307826 polymorphisms and CYP3A5 gene rs776746 polymorphisms was not significantly associated with the gender,age,tumor site,pathological type and tumor staging of patients with renal cancer in the study group.2.The incidence of wild-type homozygote at the rs307826 site of VEGFR3 gene in the region was higher than that in patients with kidney cancer in Spain.There was also a higher incidence of misphagia in the CYP3A5 gene rs776746 site than in the Spanish patients with kidney cancer.The difference between the two was statistically significant.3.There was a high degree of consistency in the distribution of VEGFR3 gene rs307826 in patients with renal carcinoma in this study(A/A type,100%).Therefore,the prediction of the therapeutic effect of sunitinib on patients with renal carcinoma in this region is not significant.The polymorphisms of the CYP3A5 gene rs776746 were different in the distribution of renal cancer patients in this study(G/G type,55.9%;G/A type,41.2%;A/A type,2.9%).There is a certain value in the prediction of the side effects of sunitinib.4.The correlation between CYP3A5 gene rs776746 variant homozygote A/A for the treatment of sunitinib is also needed to expand the sample capacity for further study and analysis.