Macrogenomics Analysis Of Gut Microbiota In Rheumatic Heart Disease

[Background and Objective]Rheumatic heart disease is a kind of autoimmune heart disease that threats human's health and life seriously.Yunnan province is located in the southwest of China and rheumatic heart disease is very popular.But poor transportation and sanitary conditions causes great difficulties for natives to have medical treatments.It has been proved that the changes of intestinal flora is related tomany autoimmune diseases.In order to find out the key bacterial components and the evidence that intestinal microflora is involved in the pathological changes of rheumatic heart disease.then identify the antibiotic resistance genes so as to understand the changes of intestinal microorganism in the period of rheumatic heart disease more comprehensively and provide help for the choice of medicine and clinical treatment.We analysis the intestinal flora of patients with rheumatic heart disease by using the high throughput sequencing technique of macrogenomics.[Method]We collected 10 stool samples from patients with rheumatic heart disease and 5 healthy controls as subjects.High fluxes were sequenced in 15 stool samples and the sequencing results,including the analysis of the structure and abundance of intestinal microflora,the annotation and analysis of KEGG database,the annotation analysis of COG database,CAZy database and ARDB database annotation,have been experience advanced bioinformatics analysis.[Result]1.The gut microbiota of the patients with rheumatic heart disease and that of the healthy control group mainly concentrated on the division level:Bacteroidetes,Firmicutes,Proteobacteria.2.The Bacteroidaceae and Bacteroides in the intestinal tract of patients with rheumatic heart disease are significantly lower than those in the healthy control group.3.The Eubacteriaceae and Eubacterium in the intestinal tract of patients with rheumatic heart disease are significantly higher than those in the healthy control group.4.The biomarker in the intestinal tract of patients with rheumatic heart disease is mainly concentrated in the Proteobacteria.5.The main differential metabolic pathways obtained from the KEGG database annotation are peptidoglycan biosynthesis and nitrogen metabolism.6.Rheumatic heart disease reduces energy metabolism in the intestine.7.The differential homologous proteins annotated in the COG database are enzymes involved in energy generation and transformation.8.The differential carbohydrate active enzyme annotated by the CAZy database is mainly concentrated on glycoside hydrolases and this hydrolase may further affect the formation of short chain fatty acids.9.Differential antibiotic resistance genes in ARDB database annotation mainly concentrated on the vancomycin resistance gene.[Conclusions]1.The biodiversity of intestinal microflora in patients with rheumatic heart disease has changed,showing the decrease of bacteroides and the increase of eubacterium.2.Under the influence of rheumatic heart disease,growth conditions of intestinal microflora's are destroyed.the energy metabolism of intestinal flora was reduced,and the metabolic functions such as peptidoglycan synthesis and nitrogen metabolism were enhanced,resulting in a more extensive metabolic pathway.3.Antibiotic resistance genes in intestinal flora of patients with rheumatic heart disease is mainly vancomycin gene,which has guiding significance for the rational use of antibiotics in clinical practice.