Imbalance Of Intestinal Of Mice Microbiota Induced By Western Diet And ApoE-deficiency:Interference By Huanglian Jiedu Decoction

BackgroundHyperlipidemia can be caused by ingestion of an excessively western diet,genetics and other factors.Previous studies have shown that ingesting western diet not only causing hyperlipidemia,but also leading to the imbalance of gut microbiota and the increasement inflammatory responses.ObjectivesWe aimed to compare the gut microbiota under western diet feeding and ApoE gene defection in mice to construct the mice model,and analyze the potential effects of Huanglian Jiedu Decoction on gut microbiota in this model.MethodsWild-type and ApoE-/-mice were fed with chow diet and western diet for 4 weeks.After collected the samples from ileum,cecum and colon feces,we performed next generation sequencing and bioinformatics analysis of 16S rDNA,and compared the different compositions in gut microbiota.Then,the group of ApoE-/-mice fed with western diet were treated with Huanglian Jiedu Decoction,Coptis Rhizoma,Berberine,Atorvastatin for 4 weeks,respectively.Their samples were also collected and sequenced to evaluate the effects of drugs to the gut microbiota.Results1.In wild-type group.the microbiota of cecum is similar to that of colon,which was significantly differed to that of ileum flora.2.Although the intake of western diet and the genetic defection with metabolic disorders can both caused the alternates of intestinal flora,the western diet showed main effects on the ileum,while the genetic defection has a broad range impacts in all of three tissues in our study.3.The ApoE-/-mice feeding with western diet significantly increased the abundance of Ruminococcus and Prevotella in the gut of mice,compared to the control.4.The interventions of Huanglian Jiedu Decoction,Coptis chinensis and Berberine can alternate the microbiota of mice with hyperlipidemia,increase the abundance of Lactobacillus and Enterobacteriaceae,and inhibit the abundance of the harmful bacteria such as Prevotella,Helicobacter and Allobaculum.ConclusionIngesting of western diet and the genetic defection can both lead to the changes of the gut microbiota in mice.Huanglian Jiedu Decoction,Coptis chinensis,and Berberine can improve the bacterial structure of mice with the hyperlipidemia.