The Differential Research On Gut Microbiota Between Patients With Parkinson's Disease And Age-matched Controls In Northeast China

objective:There is mounting evidence for a connection between the gut and Parkinson's disease(PD).Recently,non-motor symptoms are getting more and more attention and among them the constipation is one of common symptom often precede the motor symptom.Lewy body and ?-synuclein,which are neuropathological hallmarks of PD,may appear in the gut before they appear in the brain.In PD,constipation is associated with ?-synuclein accumulation and neurodegeneration in the enteric nervous system,with local increased inflammation,oxidative stress and intestinal permeability.These pathophysiological changes can occur in the initial stage of PD,and in some case,years before the motor-symptom appear.Gut microbiota,as one of the important environmental factors in the intestinal tract,may play an important role in the intestinal lesions of PD patients.This paper discusses the difference between gut microbiota of PD and healthy controls and the influencing factors of changes in gut microbiota,and finally provides new research ideas for PD treatment.Methods:A total of 51 PD and 49 healthy controls without any other disease that affect the composition of gut microbiota were involved in the study.We collected the objects' fecal at the same time and transport them to same laboratory with liquid nitrogen.Microbial species were determined through 16S-rRNA sequencing.In addition,the diet of patients,UPDRS-III,NMSQ and RBDQ-HK was investigated and the relationship between the microbiota change and diet,UPDRS-III,NMSQ and RBDQ-HK was studied.Result:We compared the difference of composition of gut microbiota between PD and healthy control.Significant difference in a-diversity were observed between T-group and C-group.The comparisons of community diversity indicated that species in T-group had less richness than C-group but had more evenness.Significant difference in?-diversity were observed between T-group and C-group.The T-test method yielded 1phylum,4 class,4 order,8 family,20 genus and 7 species.The results suggest that structural changes of gut microbiota in PD are characterized by the increases of enteric mucus-eroding and pro-inflammatory microbiota and the decrease of beneficial microbiota producing protective neurotransmitters,at the same time,the microbiota gut changes with the intake of dietary fiber has the close relation.Conclusion:There were significant changes in PD gut microbota and control group.These changes were significantly correlated with dietary fiber societies,and a balanced diet could be a new target for PD therapy.