Study On The Regulation Of Glucose Transporter Gene Expression In Human Retinal Pigment Epithelial Cells

Objective:To regulate the gene expression of glucose transporter in human retinal pigment epithelial cells.The treatment of diabetic retinopathy was studied.Method:Adopting the Crispr Cas9 system of anthropogenic retinal ARPE-19 host cells corresponding knockout,using Cas9 knock out ways to glucose transporter protein gene in the cells.Results:The human retinal arpe-19 monoclonal cell line which is glucose transporter gene knockout was built.The constructed cell line was verified by sequencing.Conclusion:We report a new strategy of using Crispr Cas9 to edit endogenous genes and possibly as a gene therapy for diabetic retinopathy.We confirmed that the design of sg RNA for glucose transporters can accurately target and target the target gene,and this treatment strategy is safe and effective in cytology.If in the future on pt.The reduction of glucose transporters technology used in the body,in the long run,using sg RNA on endogenous glucose transporter protein gene knockout therapy significance,fundamentally solves the diabetic retinopathy accumulation of glucose in the eye of all sorts of cell damage,is a potential method for the treatment of diabetic retinopathy.