| Objective: To investigate the effect of FCS wine on the development of erectile function in rats with kidney-yang deficiency and accessory sex organs and viscera index level;the mating capacity of normal rats,organ index related to reproductive development and testicular histology;protective effect on mice with kidney-yang deficiency.Methods:1.Experimental study of acute toxicity of FCS wine on mice.2.Effects of FCS wine on castrated rat's sexual excitability: SD male rats were castrated,three days later,randomly divided into six groups: model group,solvent group,positive control group,Fangcishen wine high group,medium group,and low group with ten rats in each group.Besides,ten rats without castration were selected and placed into a given normal group.Each group was medicated for 21 consecutive days.One hour after the last medication on the21 st day,a pharmacological electric device was used to stimulate sexual organs of rats while their erection was being monitored.3.Effects of FCS wine on castrated rats' reproductive system organs index:The rats as mentioned in part 2 were killed 24 hours after the last medication.Then the preputial gland,prostate gland and seminal vesicle,levator ani muscle,adrenal gland,spleen,thymus and pituitary gland were quickly removed and their indexes were calculated.4.Effects of FCS wine on rat's copulating ability: SPF-SD male rats were randomly divided into 6 groups: normal group,solvent group,positive control group,FCS-High group,FCS-Medium,and FCS-Low groups with 10 rats in each group.They are all medicated once a day for 28 consecutive days.After one hour of the last medication,the mating test was performed to record the number of times and the time that male rats lust female rats,and male rats' ejaculations.5.The effect of FCS wine on the level of SOD and MDA in plasma and testicular histology: as mentioned in part 4 above,after 24 hours of the last medication,the rats were anesthetized.Their abdominal cavities were slitted and abdomen artery's blood was drawn.Serum levels of SOD,MDA,as well as testicular histology were measured by serum isolation.6.Effect of FCS wine on normal rat's reproductive system organs index: as mentioned in part 5 above,rats were sacrificed after blood,quickly take the preputial gland,prostate and seminal vesicle,levator ani muscle and testis,accurate weighing,calculation of organ index.7.The effect of FCS wine on the impotent mice: SPF male mice were randomly divided into 7 groups: normal group,model group,solvent group,positive control group,FCS high,medium group,and low dose group,with 10 mice each.Except for the normal group,each group was given medication and injection of 25 mg / kg hydrocortisone per day for 7 days.One hour after the last medication,record the mice' weights,the number of independent activities,the swimming times,and the fatigue function.The mice will be killed after the experiment;their preputial gland,seminal vesicle + the prostate,levator ani muscle,and testis will be taken for organ index records.Results:1.Toxicity test: medicated-mice after 30 minutes to 1 hour are in unstable state and decreased in activities;the rest of the time,the mental state is good with normal activity,no significant changes in weights(P> 0.05),and no deathmice.The maximum dose of FCS for the mice was 120 mL/ kg,which was equivalent to 179.91 times the dose of human administration(0.667 mL/ kg).2.The effect of FCS wine on castrated rats: compared with the model group,the time to start the erection of the positive control,the FCS-H and the FCS-M was significantly shortened while the FCS-L group tends to be shortened.The statistical error in previous comparison was measured which P < 0.01 or P <0.05.Compared with the solvent group,the time to start the erection of rats of the FCS-H was shortened with statistical meaning of P < 0.05.3.Effect of FCS wine on castrated rats' viscera and its index :(1)Compared with the model group,the preputial gland index of the solvent and FCS-M group were significantly higher(P<0.05),the FCS-H and the FCS-L groups' prostate gland and seminal vesicle organ index was decreased significantly(P < 0.05 or P < 0.01);Compared with the solvent group,the FCS-L group's preputial gland organ index has statistical meaning(P < 0.05),the FCS-H and FCS-M groups' the preputial gland,seminal vesicle and prostate and levator ani muscle organ index had no significant difference to the solvent group(P > 0.05);(2)Compared with the model group,the thymus organ index of FCS-H were significantly lower(P < 0.05),spleen index of the FCS-L group was increased significantly(P < 0.01).Compared with the solvent group,except for the thymus index of the FCS-H group has the statistical error of P < 0.01,the pituitary,adrenal gland and spleen had no significant difference(P > 0.05).4.Effect of FCS wine on rats' copulating ability:(1)Compared with the normal group,all other medicated groups had lust and ejaculation time shortened significantly;therein,the FCS-M and FCS-L groups' lust and ejaculation times were effectively increased(P <0.01 or P <0.05);(2)Compared with the solvent group,the FCS-M and FCS-L groups' ejaculation time was shortened,but the ejaculation's numbers of time was increased,(P <0.01 or P<0.05).5.Effects of FCS wine on the blood serum SOD,MDA and testicular histology: compared with normal group,FCS-L could significantly increase the content of SOD,there was significant difference(P<0.01),FCS-M and FCS-L can decrease the content of MDA,the difference was statistically significant(P< 0.05);and solvent compared to the control group,FCS-H,FCS-M,FCS-L and positive control group could significantly increase the content of SOD,decrease the content of MDA,the difference was statistically significant(P<0.01 or P <0.05).6.Effect of FCS wine on normal rats' viscera index: compared with normal group,the FCS-L group's preputial glands index and the positive control group's prostate and seminal vesicle index were increased significantly(P<0.05),the FCS-M group's levator ani muscle index was decreased(P<0.05),the FCS-H group's thymus index was increased significantly(P<0.01).Compared with the solvent group,except for the FCS-H group's thymus index wasincreased,the FCS-L group's spleen index was decreased significantly(P<0.01 or P<0.05).Moreover,the other groups' viscera index does not have a statistically meaning(P > 0.05).7.Testicular histology: Compared with the normal group,except for the positive control and FCS-H groups' interstitial blood vessels with moderate dilation,the rest of the seminiferous tubules normal morphology,seminiferous tubule basement membrane continue the normal state;The proportion of spermatogenic cells was normal and arranged sequentially;sperms were visible;interstitial blood vessels and interstitial cells were normal.8.The effect of FCS wine on the mice' erectile dysfunction:(1)the effect Fangcishen wine on erectile dysfunction mice about their weights,independent activities and the times of swimming in low-temperature function: the normal group's weights are higher than the model group's weights(P < 0.05).The other groups had no significant difference in body weight(P> 0.05),but the independent activities and the number of time of swimming under lowtemperature were effectively increased(P < 0.01 or P < 0.05).(2)The effect of Fangcishen wine on mice' exhaustion: compared with the model group,the other groups had was no significant difference in statistic(P> 0.05).(3)Compared with the model group,the weight of the positive control group's seminal vesicle phase + prostate was increased significantly(P < 0.05);the FCS-H group's levator ani muscle was atrophied(P < 0.05),the weight of the positive control group's levator ani was increased significantly(P < 0.05)with statistical significance;the weight of testis of the three groups FCS-H,FCS-M,and FCS-L had no statistical significance.Conclusion:FCS wine obviously boots the erectile ability of castrated rats and the ejaculation of normal rats.This wine also improve the independent activity and the time of swimming in low-temperature of erectile dysfunction mice.From all of the experiments,FCS wine is proved to have effective effect on kidney and erectile dysfunction. |
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