Effect Of Xuanfei Huoxue Decoction On Pulmonary Vascular Endothelial Oxidative Injury And CLIC1 In Rats With Sepsis-induced Lung Injury

Objective To investigate the relationship between CLIC1 and pulmonary vascular endothelial injury induced by sepsis lung injury,and to observe the effect of Xuanfei Huoxue Decoction on the pulmonary vascular endothelial cell function and the expression of CLIC1 in rats with sepsis-induced lung injury.Methods Twenty-four clean-grade healthy male SD rats were randomly divided into three groups: normal control group,lung injury model group,Xuanfei Huoxue Decoction treatment group.Intraperitoneal injection of lipopolysaccharide was performed in a rat model of sepsis lung injury.Three days before modeling,Xuanfei Huoxue Decoction group Xuanfei Huoxue Decoction decoction was intragastrically administered,7.25mg/kg,2 times per day;the other two groups were given intragastric administration of normal saline,10mg/kg,2 times per day.On the 4th day: 1.In the normal control group,normal saline(NS)was injected intraperitoneally at 10 ml/kg,and after 0.5 h,physiological saline(NS,10 mg/kg)was given to the stomach;2.The lung injury model group was treated with 10mg/kg.After intraperitoneal injection of LPS in kg,0.5 h later,normal saline(NS,10 mg/kg)was given to the stomach;3.Xuanfei Huoxue Decoction treatment group was injected LPS at 10 mg/kg intraperitoneally,and after 0.5 h,it was administered at 7.25 ml/kg.Chinese medicine decoction gavage.At the end of the gavage,the specimens were collected after 6hours.Observe the pathological changes of lung tissue,detect the total protein,total cell count and middle cell count in BALF.ELISA was used to detect the expression of i NOS,ET-1,ICAM-1,VCAM-1,CLIC1 in rat lung homogenate and the content of IL-6,TNF-?,MDA and SOD in rat artery.The results of immunohistochemistry CLIC1 expression in the pulmonary artery.Result 1.Histopathological changes:Compared with the normal control group under light microscope,the alveolar in the lung injury model group was filled with edema fluid,inflammatory cell infiltration,alveolar septum widening,alveolar capillaries dilatation and congestion,and inflammatory cell infiltration.Some alveoli were compressed and collapsed.Compared with the model group,Xuanfei Huoxue decoction group had less alveolar edema,milder infiltration of inflammatory cells,less widened alveolar septum,and less dilated and congested alveolar capillaries.2.The protein content,the number of total cells and neutrophils in BALF: compared with the normal control group,the protein content,the total cell number and the number of neutrophils in the BALF model group were significantly increased,the difference was statistically significant(P<0.05);Compared with the model group,the protein content,the total number of cells and the number of neutrophils in the BALF of Xuanfei Huoxue decoction group were significantly decreased,and the difference was statistically significant(P<0.05).3.ELISA detection of serum TNF-?,IL-6 levels: The test results show that compared with the normal control group,the lung injury model group serum TNF-?,IL-6 expression was significantly higher,the difference was statistically significant Significance(P<0.05);Compared with the model group,the expression of TNF-?and IL-6 in the serum of Xuanfei Huoxue Decoction treatment group was decreased,and the difference was statistically significant(P<0.05).4.ELISA detection of serum SOD,MDA levels:The test results showed that compared with the normal control group,serum malondialdehyde(MDA)levels in the lung injury model group increased,superoxide dismutase(SOD)The activity was decreased,and the difference was statistically significant(P<0.05).Compared with the model group,the content of malondialdehyde(MDA)in serum of Xuanfei Huoxue decoction group was decreased,and the expression of superoxide dismutase(SOD)was increased.The difference was statistically significant(P<0.05).5.ELISA was used to detect the expression of i NOS and ET-1 in lung homogenate of each group: The results showed that compared with the normal control group,the expression of i NOS and ET-1 in the lung homogenate of the lung injury model group increased significantly.Statistical significance(P<0.05);Compared with the model group,the expression of i NOS and ET-1 in the lung homogenate of Xuanfei Huoxue Decoction treatment group was decreased,and the difference was statistically significant(P<0.05).6.ELISA detection of ICAM-1,VCAM-1,CLIC1 expression in lung homogenate of each group:The test results showed that compared with the normal control group,the lung tissue homogenates of lung injury model group ICAM-1,VCAM-1,The expression of CLIC1 was significantly increased(P<0.05).Compared with the model group,the expression of ICAM-1,VCAM-1and CLIC1 were decreased in the lung homogenate of Xuanfei Huoxue Decoction group,and the difference was statistically significant.Italy(P<0.05).7.Immunohistochemical detection of CLIC1 in pulmonary artery vascular tissue: Compared with the normal control group,the positive expression of CLIC1 in the acute lung injury model group was significantly higher(P<0.05);compared with the acute lung injury model group The positive expression of CLIC1 in Xuanfei Huoxue decoction group decreased,and the difference was statistically significant(P<0.05).Conclusion 1.Rats with lung injury of sepsis can be successfully duplicated by intraperitoneal injection of LPS;2.Oxidative stress and release of inflammatory factors in ALI rats with sepsis;3.The expression of CLIC1 in pulmonary vascular endothelium in sepsis ALI rats.4.Increased vascular endothelial dysfunction in ALI rats with sepsis.5.Xuanfei Huoxue Decoction may reduce oxidative stress,reduce inflammation,and inhibit the expression of pulmonary vascular endothelial CLIC1,thereby improving endothelial function and reducing lung tissue damage.