MicroRNA Expression In Patients With Chronic Arrhythmia

[background] Arrhythmia is one of the most serious disease of cardiovascular disease.It is also the most common clinical cardiovascular disease.In recent years,the incidence of slow arrhythmia is increasing,and its pathogenesis has also attracted people's attention.At present,the standard of clinical treatment of slow arrhythmia treatment plan for implantation of artificial pacemakers,the clinical applications and clinical history of cardiac pacing is more than 50 years,the structure and function of today's artificial pacemakers have Reached a very high level,but the implantation of electronic pacemaker still has some insurmountable defects and problems.In recent years,the study of microRNAs in cardiovascular diseases has become more and more thorough.The expression of miR-1,miR-133 and let-7 are extremely abundant in cardiomyocytes.And microRNAs such as miR-21 and miR-208 are closely related to the occurrence of atrial fibrillation and arrhythmia.However,there are few microRNA studies on bradyarrhythmia.In recent years,the incidence of bradyarrhythmia has been increasing,and its lack of effective early warning diagnosis and markers.[Objective] Comparing the expression of microRNA-1,microRNA-21,microRNA-133,microRNA-208 in patients with bradyarrhythmia and healthy subjects with U6 as reference,and observe the he relationship between microRNA and bradyarrhythmia,and explore the association of microRNAs with slow arrhythmias.[Methods] 1?Collecting the fresh blood of the patients with bradyarrhythmia and healthy,and divide the collected blood into three groups,sick sinus syndrome group(SSS group),.atrioventricular block group(AVB group)and health group(H group).2?Extracted RNA from each group With the columnar method,after cleavage of red blood cells,phase separation,precipitation and washing steps.3?The extracted RNA was measured by UV spectrophotometer concentration and purity(A260 / A280).4?the obtained RNA products were reverse transcribed into cDNA by adding the microRNA-1,microRNA-21,microRNA-133,microRNA-208 and microRNA-U6 as primers.5?use pcr instrument to amplify the cDNA by real-time pcr to detect the CT value of the amplified product(the number of cycles that each fluorescence signal in the reaction tube reaches the set threshold value),and detect the microRNAs in each group,compare with the health group,and calculate the relative expression and analyzed statistically.[Results](1)Compared with H group,the expression of microRNA-21 in SSS group was reduced to 13.2%(P <0.05).There was no significant difference between the expression level of other microRNA-1,microRNA-133,microRNA-208 and the health group.(P> 0.05).(2)Compared with H group,the number of microRNA-1 in AVB group decreased to 18.7%,the number of microRNA-21 decreased to 7.3% and the number of microRNA-208 decreased to 22.2%(P <0.05)There was no significant difference between the microRNA-133 and the health group.(P> 0.05).[Conclusions] 1? In sick sick sinus syndrome patients,microRNA-21 significantly decreased.2 ? In atrioventricular block patients,microRNA-1,microRNA-21,microRNA-208 decreased significantly.