Exploration Of The Anti-tumor Effects And Mechanisms Of Chrysomya Megacephala(Fab.)and Extract In H22 Tumor-bearing Mice

Objective:To investigate the anti-tumor effects and mechanisms of Chrysomya megacephala(Fab.)and extracts in H22 tumor-bearing mice.Methods:H22 solid tumor bearing mice models were established and mice were randomly divided into seven groups:the normal group,model group,CTX group?Chrysomya megacephala(Fab.)low,high dosage group(0.75,1.5 g/kg)and Chrysomya megacephala(Fab.)Extracts low,high dosage group(0.75,1.5g/kg).Each group was given corresponding drug by gavage for 10 days.The tumor growth situation of mice was observed and the tumor inhibition rate was calculated.Blood routine examination and liver and kidney function index of mice were determined.The expressions of p38MAPK and phosphorylated p38MAPK in tumors were detected by Western blotting.The levels of IL-1?,IL-6,TNF-?,and VEGF in tumors were detected by ELISA.H22 solid tumor bearing mice models were reconstructed,and experimental conditions is the same as before,expect medication groups change into Chrysomya megacephala(Fab.)Extracts low,middle,high dosage group(0.375,0.75,1.5 g/kg);After ten days treatment,mice were sacrificed,and tumor,thymus and spleen were weighed immediately.The immune organ indexes and the rate of tumor inhibition were calculated.The levels of IL-2,IFN-?and Ig G in tumorstissue were detected by ELISA.The number of CD3~+,CD4~+,CD8~+lymphocyte cell were counted by flow cytometry.Results:There was no anti-tumor effect in Chrysomya megacephala(Fab.)dosage groups.The tumor growth of H22 tumor-bearing mice were inhibited in CTX group and Chrysomya megacephala(Fab.)Extracts 0.75,1.5 g/kg dosage group(P<0.05).There was repeatability on result of tumor inhibition.Chrysomya megacephala(Fab.)Extracts has no hepatorenal damage.The protein expression of p-p38MAPK in each Chrysomya megacephala(Fab.)Extracts dosage group was higher than that in the model group(P<0.05).ELISA results showed that levels of IL-1?,IL-6,TNF-?in Chrysomya megacephala(Fab.)Extracts 0.75 g/kg,1.5 g/kg dosage group were higher than that in the model group,and the level of VEGF in 0.75 g/kg,1.5 g/kg dosage group were lower than that in the model group(P<0.05).There is no effect on immune organ weight and indexes after Chrysomya megacephala(Fab.)Extracts treatment,the counts of CD3~+cell of each group were not affected by Chrysomya megacephala(Fab.)Extracts(P>0.05),but Chrysomya megacephala(Fab.)Extracts can improve the ratio of CD4~+/CD8~+in peripheral bloodand spleen of H22 tumor-bearing mice(P<0.05).ELISA results showed that Chrysomya megacephala(Fab.)Extracts have no influence on the level of IL-2 in tumor tissue(P>0.05),the level of IgG in 0.375 g/kg,0.75 g/kg dosage group were lower than that in the model group(P<0.05),the level of IFN-?in tumor issue was improved significantly in Chrysomya megacephala(Fab.)Extracts 0.75g/kg dosage group(P<0.05).Conclusion:Chrysomya megacephala(Fab.)have no inhibitory action on tumor in 0.75?1.5 g/kg dosages of medication.Chrysomya megacephala(Fab.)Extracts in the dosages of 0.75?1.5 g/kg can inhibit H22 tumor growth in mice,and it was repeatability on result of tumor inhibition.the underlying mechanism may be related to the mediation of some cytokines and the activation of p38MAPK signal pathway,regulate the mice'specific immune function of T cells and improve the level of IFN-?in the tumor tissue.