| Objective: To study the effect of aqueous extract of Zebrina pendula Schnizl.(AEZPS)on glucose and lipid metabolism,oxidative stress and inflammatory injury in the streptozotocin(STZ)-induced diabetic mice.Methods: Male mice were treated with STZ by tail vein injection(0.12g/kg body weight)and were confirmed as diabetic mouse models when the level of blood glucose was ?11.1 mmol/L.Subsequently,STZ-induced diabetic mice were treated intragastrically with AEZPS(10,20 and 40 g/kg body weight/d)and metformin(0.32 g/kg body weight/d)for 14 days.At the same time,13 healthy mice were used as blank control group.The model control group and the control group were fed with double distilled water(20 mL/kg)for 14 days.The change of the weight in mice was observed and recorded every two days.And fasting blood glucose was determined every week.1.The effect of AEZPS on glucose and lipid metabolism: all mice were killed at the 15 th day,the levels of fasting blood glucose(FBG),oral glucose tolerance test(OGTT),fasting serum insulin(FINS),total cholesterol(TC)and triglycerides(TG)were measured.In addition,the pathological analysis ofpancreas was measured by hematoxylin and eosin(HE)staining.2.The effect of AEZPS on oxidative stress: the levels of malondialdehyde(MDA),superoxide dismutase(SOD)and glutathione peroxidase(GSH-PX)in the serum and liver tissue were measured by commercial kits.3.The effect of AEZPS on inflammatory injury: interleukin-6(IL-6)and tumor necrosis factor-?(TNF-?)in the serum were measured by commercial kits.The pathological analysis of liver tissue were measured by HE staining.The expressions of toll-like receptor 4(TLR4),myeloid differentiation factor 88(MyD88)and nuclear-factor kappa B(NF-?B)were measured by immunohistochemistry.In addition,the mRNA levels of MyD88 and NF-?B were measured by fluorescence quantitative-polymerase chain reaction(FQ-PCR).Results: 1.The effect of AEZPS on glucose and lipid metabolism:compared with the model group,the administration of AEZPS(20 and 40 g/kg body weight/d)or metformin to diabetic mice resulted in a significant increase in body weight(P<0.05),and AEZPS or metformin treatment markedly attenuated hyperglycemia in diabetic mice(P<0.05).The blood glucose area under the curve and TG were lower than those in the model group,but the levels of FINS were increased in AEZPS-H,AEZPS-M and metformin group(P<0.05).And the levels of TC were reduced in AEZPS and metformin group(P<0.05).In addition,compared with STZ model control group,HE staining showed that pancreatic pathology damage was reduced and islet ? cells were protected in AEZPS and metformin group.2.The effect of AEZPS on oxidative stress: compared with the model group,the serum levels of MDA in the AEZPS-and metformin-treated group were significantly lower than those in the model group(P<0.05),the serum contents of GSH-PX in the AEZPS-and metformin-treated group were significantlyhigher(P<0.05),SOD in the serum was increase in AEZPS groups except the low group one(P<0.05).In liver tissue,the levels of MDA in the AEZPS-and metformin-treated group were significantly lower than those in the model group(P<0.05),and the levels of SOD and GSH-PX in high dose and middle dose experimental group were significantly higher than those in the model group(P<0.05).3.The effect of AEZPS on inflammatory injury: compared with the model group,the levels of IL-6 and TNF-? in AEZPS-and metformin-treated group were significantly decreased(P<0.05),the injury of liver in the treated group was less than that in the model group,the expressions of TLR4,MyD88 and NF-?B protein in liver tissue were lower,and the treatment in metformin group,high dose experimental group and middle dose experimental group,led to a decline in the mRNA expression levels of MyD88 and NF-?B(P<0.05).Conclusion: AEZPS may act as a new therapeutic for diabetes by the regulation of glucose and lipid metabolism,improving oxidative stress and suppressing inflammation damage. |
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