| Objective:To explore the experimental method of MRI-pathologic correlation of Imaging-to-Section,ROI-to-Site on simulated soft tissue mass model.Materials and Methods:1.Experimental equipment(1)The raw material for making simulated limbs and trunk soft tissue mass model is polyacrylamide pyrrolidone,methylcellulose,polymer polyol and distilled water.The production equipment mainly includes a heat-collecting electromagnetic heating stirrer and a low-temperature test chamber.The model is colored with natural water-based color paste.(2)Making rabbit soft tissue simulated mass animal models using live rabbits,simulate mass using a fresh chicken heart.(3)Model image examination using Affiliated Second Hospital of DaLian Medicine University Radiology 3T superconducting MR.(4)Model positioning using vitamin AD capsules,measurement of tumor model section length using a scale.2.Experimental method(1)The production method of simulated limbs soft tissue mass model and simulated trunk soft tissue mass model.The distilled water is placed in a heat-collecting electromagnetic heating stirrer heated to 45?,the polyacrylamide pyrrolidone,methyl cellulose,polymer polyol according to a certain percentage added to distilled water at 85? to 100? under reaction 4 to 5 Hour,fully integrated into a polymer.The polymer was dyed with a natural water-based color paste.The dyed polymer was injected into a four-limbed mold made of silicone rubber,a skin muscle mold and a mass mold respectively.After standing for half an hour,the bubbles were removed and placed in a low-temperature chamber(-41?)Frozen in cross-linked shape,removed after 8 hours,thawed at room temperature for 2 hours.The mass model into the limbs model and skin muscle model,with a small amount of polymer as a coupling agent,static 2 hours after the curing agent.(2)The production method of rabbit soft tissue simulated mass animal modelLive rabbits were anesthetized with 10% chloral hydrate by intraperitoneal injection at a dose of 3.5 ml / kg.The rabbits were completely anesthetized using tissue scissors to trim the hair around the right thigh of the rabbits.The pruning area was about 5*5 cm.A scalpel was used to make a subcutaneous incision at the edge of the clean hair trimmed area.The incision was about 2.5 cm in length.Into the skin,suture incision.(3)The method of radiology pathology correlation in modelMass positioning in surface: palpate the mass,the mass within the model to get the approximate range of the mass in the middle position with a horizontal scale and marker strokes positioning a line for the body surface positioning line(PL),the first line.The body positioning line is vertical to the model.Three glue sticks of Vitamin AD(for short three point)are used as local markers(LM)on the body surface positioning line.MR examination: examine the labeled model after mass positioning,first of all conventional MRI sagittal or coronal T2 WI examination to determine the extent of the mass,sagittal or coronal images obtained after the image of the model to find the surface markers,and then cross-section T1 WI,T2WI examination,cross-sectional examination line parallel to the surface of the sagittal or coronal images multiple positioning markers located in the line to obtain parallel to the alignment line MRI cross-sectional images corresponding to the three models were the image A.Mass positioning during operation: The scalpel along the longitudinal axis of the incision,carefully peel the surrounding tissue,exposing the mass,along the surface of the body positioning line in the direction of the center of exposure to wear a surgical line,line A,the second line;Along the direction of body surface positioning line in the right side of the tumor exposed surface wear a surgical line for the line B,the third line;along the direction of the body surface positioning line exposed on the left side of the tumor wear a surgical line for the line C,the fourth line,line A,line B,line C are in the mass,and in a straight line,and the body surface alignment line coincides with the last of the exposed tumor in the upper longitudinal wear a line as the direction of the line,line D,the fifth line.Last free mass,remove carefully.Mass positioning of pathological tissue selected section and site: according to the line D and the line A,line B,line C positioning,find the mass up and down direction according to the line D,find the left and right direction of the mass according to the line A,Line B,line C,placed in line with the position of the mass in the model consistent with the scalpel according to line A,line B,line C of the face of large incision,continue to do parallel to the section of the pathological large section according to MR check layer thick,the model were obtained with the image A pathological general section,respectively,section A in the cross-sectional MRI image selected a square area,In the corresponding section to find the area,pathological material.3.Observed indexes(1)Measure the longest diameter and the shortest diameter perpendicular of the longest diameter of simulated mass in the image A.(2)Use the scale to measure the maximum diameter and the minimum diameter perpendicular to maximum diameter of the simulated mass in the section A.The above values were measured three times the average.4.Statistical Analysis:(SPSS 24.0 software)The paired t-test was used to calculate the difference of longest diameter in the image A and maximum diameter in section A of simulated mass.The paired t-test was used to calculate the difference of shortest diameter in the image A and minimum diameter in section A of simulated mass.Results:1.Differences between the values of M1 and M2,N1 and N2 in 10 simulated limbs soft tissue mass models(1)There was no statistical significance(P> 0.05)difference between M1 and M2 in 10 simulated limbs soft tissue mass models,the 95% confidence interval of the difference was-0.03585-0.02785,the t was-0.284,the P was 0.783.(2)There was no statistical significance(P> 0.05)difference between N1 and N2 in 10 simulated limbs soft tissue mass models,the 95% confidence interval of the difference was 0.02485~0.03285,the t was-0.314,the P was 0.761.2.Differences between the values of M3 and M4,N3 and N4 in 10 simulated trunk saft tissue mass models(1)There was no statistical significance(P> 0.05)difference between the M3 and M4 in 10 simulated trunk saft tissue mass models,the 95% confidence interval of the difference was-0.03451-0.04451,the t was-0.286,the P was 0.781.(2)There was no statistical significance(P> 0.05)difference between N3 and N4 in 10 simulated trunk saft tissue mass models,the 95% confidence interval of the difference was-0.03826-0.03026,the t was-0.264,the P was 0.798.3.Differences between the values of M5 and M6,N5 and N6 in 3 rabbits soft tissue simulated mass of animal models(1)There was no statistical significance(P> 0.05)difference between M3 and M4 in the rabbit soft tissue simulated mass of 3 animal models,the 95% confidence interval of the difference was-0.00023-0.08223,the t was 2.250,the P was 0.051.(2)There was no statistical significance(P> 0.05)difference between N5 and the in the rabbit soft tissue simulated mass of 3 animal models,the 95% confidence interval of the difference was-0.00770-0.06770,the t was 1.8000,the P was 0.105Conclusions:Through three groups of model experiments: simulated limbs soft tissue mass model,simulated trunk soft tissue mass model and rabbit soft tissue simulated mass model,we get the conclusion:1.The method of three-points and five-lines of MRI-pathology in simulated soft tissue mass model was proposed for carry out MRI-pathologic correlation studies in it at first time,and it is highly reproducible.2.In the three groups of simulated limbs soft tissue mass model,simulated trunk soft tissue mass model and rabbit soft tissue simulated mass model,the MR imaging can accurately correspond to the pathological section.The image ROI accurately corresponds to the site of the pathology section,and then obtain accurate MRIPathologic correlation information lays a solid foundation for future clinical studies on soft tissue tumor MRI-pathologic correlation. |
PDF Full Text Request