Study On Chemical Constituents From Biluochun Tea From Suzhou Dongting Mountain

Tea is the leaves of the plant which is a member of Theaseae family,and its another name is tu or ming,etc.Tea is the second most consumed beverage in the world besides water,surpassing coffee,beer and carbonated drinks.In addition to drink,there are many other uses for tea,such as medicine and tanning materials,which have been proved to be much earlier than drinking.As early as in the tang dynasty,there were recorded in the new grass: "tea,bitter tea,sweet taste,bitter,mild cold and non toxic,the main fistula sore,liurinate,to be thirsty,to sleep less,the lord is down".In the Ming dynasty,compendium of materia medica written by li shizhen is also recorded: leaves,bitter,sweet,mild cold,non toxic.Main treatment fistula ulcer,urinate,go to phlegm fever,quench thirst,make sleep less,powerful,happy...The warm drink is reduced by the cold,and the hot drink is broken up by the fire.It also serves as a refreshing drink,which makes you feel good and not drowsy.In addition,in other important materia medica books,there have detailed records of tea and taken together,the tea has the effect of a little sleep,thirst thirst,qingrejiedu cure sore fistula etc,with the modern scientific experiments prove that the tea has excited refreshing,diuresis,anti-inflammatory sterilization effect is very consistent.At present,FDA has only approved two listed botanical drugs.One of them is tea polyphenols approved by the US FDA in October 2006.It is used as a prescription drug for local treatment of human papillomavirus induced genital warts.However,the wide medicinal value of tea is still not fully understood and explored,so tea has research and development value.Biluochun Tea is one of the top ten famous teas in China,and its another is xiangxiarenxiang.It belongs to green tea with 1000 years history.Biluochun tea is a special product in suzhou.Its shape is slender,and its hair is all over.After brewing tea,the color is bright green and bright,the flavor is rich and fragrant,and the drink has a feeling of sweetness.The unique flower fruit of the Biluochun Tea is mainly because it grows in the orchard,and it is nourished by the unique soil and water of the dongting.Biluochun tea was researched mostly concentrated in the aroma composition,catechins and flavonoids,and for other water-soluble fraction,biluochun tea?s composition is reported for the first time.In order to explore the pharmacodynamic material basis of biluochun tea and to study its active ingredients to improve its material basis,this research group studied the chemical composition of biluochun tea from xishan in suzhou.The chemical constituents were isolated and purified by repeated silica gel column chromatography,Sephadex LH-20 gel column chromatography,ODS column chromatography,high pressure flash chromatography and semi-preparative HPLC,etc.Finally 30 compounds were elucidated on the basis of physico-chemical constants and spectral analysis.That was caffine(LT-1)?protocatechuic acid(LT-2),caffeic acid(LT-3)?3-methoxy 4,5-dihydroxy-benzoic acid(LT-4)?tricin(LT-5)?quercetin(LT-6)?astra LTlin(LT-7)?luteolin(LT-8)?kaempferol-3-O-?-glucopyranosyl(1?2)-?-glucopyranoside-7-O-?-rhamnopyranoside(LT-9)?Camellikaempferoside A(LT-10)?Camellikaempferoside C(LT-11)?Camelliquercetiside B(LT-12)?Camelliquercetiside A(LT-13)? Camelliquercetiside G(LT-14)? Chakaflavonoside A(LT-15)?Camelliquercetiside C(LT-16)?pomonic acid(LT-17)?pomolic acid(LT-18)?ursolic acid(LT-19)? 3-O-?-L-arabinopyranosyl pomolic acid(LT-20)?3?-[(?-L-arabinopyranosyl)oxy]-urs-12,19(20)-dien-28-oic acid(LT-21)?3-O-?-L-arabinopyranosides of oleanolic acid(LT-22)?3?-[(?-L-arabinopyranosyl)oxy]-urs-12,18-dien-28-oic acid(LT-23)?21?-hydroxyl-pomolic acid(LT-24)?20-epi-urs-12,18-dien-28-oic acid 3?-O-?-L-arabinopyranoside(LT-25)?(-)-LTllocatechin 3-O-LTllate(LT-26)?(-)-epi LTllocatechin 3-O-LTllate(LT-27)?(-)-epicatechin 3-(3-O-methyl)LTllate(LT-28)?catechu alkaloid A(LT-29)?catechu alkaloid B(LT-30).The coumponds LT-14?LT-24?LT-29?LT-30 are novel coumponds,LT-5?TL-9?TL-17?TL-18?TL-19?TL-20?TL-21?TL-22?TL-23?TL-25 were firstly isolated from the genus Theaseae.This paper further studied chemical composition from the water-soluble fraction,which enriched suzhou specialty biluochun tea?s material foundation,and it is convenient for later to develop and research to the medicinal value of biluochun tea.