Hericium Erinaceus Preparation Antibacterial Extracts And Its Active Ingredients Research

Helicobacter pylori infection was one of the most common pathogens for humans,chronic gastritis,duodenal peptic ulcer main cause of the diseases of the stomach and duodenum,which was closely related to the incidence of gastric cancer induced.Hericium erinaceus was famous for Food and Medicine fungi,anti-ulcer,anti-inflammatory,anti-tumor,anti-aging effect.the Hericium piece made from raw materials of Hericium erinaceus solid mycelium alone or as a secondary drug,which for digestive diseases of chronic gastritis,duodenal ulcer,12 ulcerative colitis,gastrointestinal cancer and stomach cancer had have a good effect.This article was on the basis of previous studies,by trackinginhibit H.pylori active substances in Hericium Preparation,Purification and research activity.This thesis incudes six chapters consist of three parts as follows:1.Hericium erinaceus inhibit H.pylori activity of extract preparation of new technology This section is intended macroporous resin separation of Hericium solid fermentation mycelium inhibition of H.pylori active component preparation process,the use of active tracking method,compared HPD-100,HPD-400,D101,AB-8 fourkinds of large adsorption resin mycelia of Hericium inhibit Helicobacter pylori adsorption and desorption properties of the active ingredient,screened better separation and purification of HPD-100 experimental study.Experiments show that HPD-100 macroporous resin adsorption and desorption of the active chemical components of the inhibition of Helicobacter pylori,and to determine the inhibition of Helicobacter pylori active substance is mainly concentrated in the 50-95%ethanol eluent,activity prior to separation.the MIC:10000-20000μg/mL improve to MIC:125 to 250μg/mL 80.2.Hericium erinaceus preparation Purification and inhibit H.pylori activity This part of the antibacterial activity tracking methods,by preparation of Hericium erinaceus saponification reaction,then after organic solvent extraction into diethyl ether,petroleum ether,chloroform and n-butanol four parts,and then purified by silica gel chromatography,gel filtration chromatography,the recrystallization and nuclear magnetic resonance spectroscopy technology separation and purification and identification of six compounds,and the newly discovered one new compounds compounds:HE-1(unknown),,the others compounds were HE-2(4-Chloro-3,5-dimethoxybenzylalcohol)、HE-3(7α,8β,11-Trihydroxydrimane)、HE-5(1-(5-chloro-2-hydroxyphenyl)-3-Methyl-1-butanone)、HE-6(4-Chloro-3,5-dimethoxybenzoic acid)、HE-8(3-Acetyl-4-methoylbenzoic acid).The in vitro antibacterial test:HE-1,HE-5 and HE-6 for inhabit H.pylori ATCC43504 minimum inhibitory concentration(MIC)separation for 50 to 100μg/mL,31.25 to 62.5μg/mL and 125 to 250μg/mL.Simultaneously HE-1,HE-5.3.H.pylori resistance molecular mechanisms and inhabited The part of the 96-well plates to metronidazole,clarithromycin,levofloxacin,tetracycline and determination of minimum inhibitory concentration MIC identified clinical H.pylori,of which the strain HpZL,Hp3 and HP9 resistant to metronidazole resistance,drug average 64μg/mL;HpW2504 and Hp W to metronidazole,clarithromycin and levofloxacin with the multi-drug resistance,drug levels were 128μg/mL,8μg/mL and 16μg/mL.Resistance by the rdxA and frxA,23 S rRNA,gyrA and 16S rDNA gene control.The design of these gene primers for PCR and sequencing,and then the by BioEdit comparison method,the analysis of the sites of mutations in these genes in the resistant strains of H.pylori.Hericium erinaceus preparation HE-1,HE-5 and the HE-6 the resistant strains HpW2504 and the HpW minimum inhibitory concentration MIC were 250μg/mL,100 to 200μg/mL,31.25 to 62.5μg/mL,31.25 to 62.5μg/mL,these compounds resistant strains,which provides the basis for the future development of new drugs.